Gap junction permeability is diminished in proliferating vascular smooth muscle cells.
Gap junction permeability is diminished in proliferating vascular smooth muscle cells.
复制标题
增殖的血管平滑肌细胞的间隙连接通透性降低。
DOI：
10.1152/ajpcell.1998.275.6.c1674
复制
发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Burt,JM
中科院分区：
文献类型：
--
作者：
Kurjiaka,DT;Steele,TD;Olsen,MV;Burt,JM
在动脉粥样硬化和高血压中，血管平滑肌细胞（SMC）受到刺激增殖并表现出增强的间隙连接蛋白表达。我们的目标是确定增殖与生长停滞的 SMC 之间的间隙连接功能是否存在差异。 A7r5 细胞（胚胎大鼠主动脉 SMC）在血清含量减少的培养基中不增殖（在 1% 胎牛血清中 48-96 小时后，约 90% 的细胞处于 G0/G1 期）。在增殖与生长停滞的 A7r5 细胞中，染料耦合较少，但电耦合相当，表明连接选择性通透性存在差异。在生长停滞的细胞中，用谷氨酸钾、四乙基氯化铵和 KCl 测量的连接电导可以通过这些溶液的电导率很好地预测。相比之下，在增殖细胞中用谷氨酸钾和氯化四乙铵测量的连接电导明显大于这些溶液的电导率所预测的。这些结果表明，生长停滞细胞之间的连接处被阴离子和阳离子同样良好且同时地渗透，而增殖细胞之间的连接处被任一电荷的大分子渗透性较差，而小阴离子和阳离子同样良好但不同时地渗透。数据表明，A7r5 细胞独立于电耦合调节化学耦合，这种能力可以促进生长控制，同时保护血管的血管舒缩反应性。
In atherosclerosis and hypertension, vascular smooth muscle cells (SMCs) are stimulated to proliferate and exhibit enhanced gap junction protein expression. Our goal was to determine whether gap junction function differs in proliferating vs. growth-arrested SMCs. A7r5 cells (embryonic rat aortic SMCs) did not proliferate in media with reduced serum (∼90% of cells in G0/G1phase after 48–96 h in 1% fetal bovine serum). Dye coupling was less but electrical coupling was comparable in proliferating vs. growth-arrested A7r5 cells, suggesting differences in junctional permselectivity. In growth-arrested cells, junctional conductances measured with potassium glutamate, tetraethylammonium chloride, and KCl were well predicted by the conductivities of these solutions. In contrast, junctional conductances measured with potassium glutamate and tetraethylammonium chloride in proliferating cells were significantly greater than predicted by the conductivities of these solutions. These results suggest that junctions between growth-arrested cells are permeated equally well and simultaneously by anions and cations, whereas junctions between proliferating cells are poorly permeated by large molecules of either charge and equally well but not simultaneously by small anions and cations. The data indicate that A7r5 cells regulate chemical coupling independent of electrical coupling, a capacity that could facilitate growth control while protecting vasomotor responsiveness of vessels.
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影响因子：
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作者：
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DOI：
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期刊：
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影响因子：
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DOI：
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影响因子：
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DOI：
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DOI：
--
发表时间：
1993
期刊：
影响因子：
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