Interleukin 6 decreases cell-cell association and increases motility of ductal breast carcinoma cells.
Interleukin 6 decreases cell-cell association and increases motility of ductal breast carcinoma cells.
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白介素6减少细胞细胞的缔合并增加导管乳腺癌细胞的运动。
DOI：
10.1084/jem.170.5.1649
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发表时间：
1989-11-01
影响因子：
15.3
通讯作者：
SEHGAL, PB
中科院分区：
文献类型：
--
作者：
TAMM, I;CARDINALE, I;KRUEGER, J;MURPHY, JS;MAY, LT;SEHGAL, PB
用IL-6处理转化的乳腺导管上皮细胞产生独特的细胞表型，其特征在于增殖减少和运动性增加。人导管癌细胞（T-47 D和ZR-75-1系）通常为上皮样形状，并在培养物中形成致密集落。延时电影显微摄影显示，一些未经处理的细胞可以暂时变成梭形或星状形状，并在菌落内彼此分离，但它们通常会重新加入它们的邻居。虽然IL-6抑制这些癌细胞的增殖，但IL-6处理的细胞通常变成星状或梭形，并显示出增加的运动性。只要细胞暴露于IL-6，这些变化就会持续存在。这导致细胞在集落内分散。去除IL-6后，对细胞生长、形状和运动性的影响是可逆的。IL-6处理的T-47 D细胞显示减少的粘附分子型细胞连接，如通过显著减少的含粘着斑蛋白的粘附和细胞间桥粒附着所指示的。对ZR-75-1细胞形状、集落数和DNA合成的影响取决于IL-6浓度，范围为0.15 - 15 ng/ml。在T-47 D细胞中需要更高的浓度以获得等效效果。抗IL-6免疫血清阻断IL-6作用。IL-6代表了一种充分表征的分子，其调节乳腺导管癌细胞的增殖和连接形成能力。
Treatment of transformed breast duct epithelial cells with IL-6 produces a unique cellular phenotype characterized by diminished proliferation and increased motility. Human ductal carcinoma cells (T- 47D and ZR-75-1 lines) are typically epithelioid in shape and form compact colonies in culture. Time-lapse cinemicrography shows that some untreated cells can transiently become fusiform or stellate in shape and separate from each other within a colony, but they usually rejoin their neighbors. While IL-6 suppresses the proliferation of these carcinoma cells, the IL-6-treated cells generally become stellate or fusiform and show increased motility. These changes persist as long as the cells are exposed to IL-6. This results in the dispersal of cells within colonies. The effects on cell growth, shape, and motility are reversible upon removal of IL-6. IL-6-treated T-47D cells display diminished adherens-type cell junctions, as indicated by markedly decreased vinculin-containing adhesions and intercellular desmosomal attachments. The effects on ZR-75-1 cell shape, colony number, and DNA synthesis are dependent on IL-6 concentration in the range from 0.15 to 15 ng/ml. Higher concentrations are required in T-47D cells for equivalent effects. Anti-IL-6 immune serum blocks IL-6 action. IL-6 represents a well-characterized molecule that regulates both the proliferation and junction-forming ability of breast ductal carcinoma cells.
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DOI：
10.1083/jcb.102.5.1843
发表时间：
1986-05
期刊：
The Journal of cell biology
影响因子：
--
作者：
Drenckhahn D;Franz H
通讯作者：
Franz H
DOI：
10.1073/pnas.83.10.3302
发表时间：
1986-05-01
影响因子：
11.1
作者：
LIOTTA, LA;MANDLER, R;SCHIFFMANN, E
通讯作者：
SCHIFFMANN, E
影响因子：
64.8
作者：
HIRANO, T;YASUKAWA, K;KISHIMOTO, T
通讯作者：
KISHIMOTO, T
DOI：
10.1016/s0006-291x(88)80043-3
发表时间：
1988-11-30
影响因子：
3.1
作者：
GANAPATHI, MK;MAY, LT;KUSHNER, I
通讯作者：
KUSHNER, I
影响因子：
7.8
作者：
PASDAR, M;NELSON, WJ
通讯作者：
NELSON, WJ