Genome sequence of Xanthomonas fuscans subsp. fuscans strain 4834-R reveals that flagellar motility is not a general feature of xanthomonads.
Genome sequence of Xanthomonas fuscans subsp. fuscans strain 4834-R reveals that flagellar motility is not a general feature of xanthomonads.
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Xanthomonas fuscans亚种的基因组序列。 Fuscans菌株4834-R揭示了鞭毛运动并不是黄褐色的一般特征。
DOI：
10.1186/1471-2164-14-761
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发表时间：
2013-11-06
期刊：
影响因子：
4.4
通讯作者：
Jacques MA
中科院分区：
文献类型：
--
作者：
Darrasse A;Carrère S;Barbe V;Boureau T;Arrieta-Ortiz ML;Bonneau S;Briand M;Brin C;Cociancich S;Durand K;Fouteau S;Gagnevin L;Guérin F;Guy E;Indiana A;Koebnik R;Lauber E;Munoz A;Noël LD;Pieretti I;Poussier S;Pruvost O;Robène-Soustrade I;Rott P;Royer M;Serres-Giardi L;Szurek B;van Sluys MA;Verdier V;Vernière C;Arlat M;Manceau C;Jacques MA
黄单胞菌是与植物相关的细菌，对经济上重要的作物的疾病负责。褐色黄单胞菌亚种镰刀菌（Xff）是菜豆细菌性枯萎病的病原之一。本研究测定了Xff 4834-R菌株的全基因组序列，并与其他黄单胞菌的全基因组序列进行了比较。比较基因组学分析揭示了Xff 4834-R与其他黄单胞菌共有的核心特征，包括趋化元件、双组分系统、TonB依赖性转运蛋白、分泌系统（从T1 SS到T6 SS）和多效应子。例如，预测了具有两个转录激活因子样效应子的29个3型效应子（T3 E）的库。与其他黄单胞菌基因组相比，移动的元件与基因组结构和基因内容的主要修饰相关。值得注意的是，33 kbp的缺失影响Xff 4834-R中鞭毛的生物合成。一个完整的鞭毛簇的存在下进行了评估，在收集的300多株代表不同物种和致病型的黄单胞菌。5%的测试菌株在鞭毛簇中呈现缺失并且是非运动的。此外，与4834-R相比，从同一流行病中分离的Xff菌株中有一半是非运动的，并且该比例在下一代菜豆种子世代的菌株中是保守的。这项工作描述了第一个基因组的黄单胞菌菌株致病豆和报告存在的非运动的黄单胞菌属于不同的物种和致病变种。从自然流行病中分离出这样的Xff变体可能表明鞭毛运动性不是植物适应性的关键功能。
Xanthomonads are plant-associated bacteria responsible for diseases on economically important crops. Xanthomonas fuscans subsp. fuscans (Xff) is one of the causal agents of common bacterial blight of bean. In this study, the complete genome sequence of strain Xff 4834-R was determined and compared to other Xanthomonas genome sequences. Comparative genomics analyses revealed core characteristics shared between Xff 4834-R and other xanthomonads including chemotaxis elements, two-component systems, TonB-dependent transporters, secretion systems (from T1SS to T6SS) and multiple effectors. For instance a repertoire of 29 Type 3 Effectors (T3Es) with two Transcription Activator-Like Effectors was predicted. Mobile elements were associated with major modifications in the genome structure and gene content in comparison to other Xanthomonas genomes. Notably, a deletion of 33 kbp affects flagellum biosynthesis in Xff 4834-R. The presence of a complete flagellar cluster was assessed in a collection of more than 300 strains representing different species and pathovars of Xanthomonas. Five percent of the tested strains presented a deletion in the flagellar cluster and were non-motile. Moreover, half of the Xff strains isolated from the same epidemic than 4834-R was non-motile and this ratio was conserved in the strains colonizing the next bean seed generations. This work describes the first genome of a Xanthomonas strain pathogenic on bean and reports the existence of non-motile xanthomonads belonging to different species and pathovars. Isolation of such Xff variants from a natural epidemic may suggest that flagellar motility is not a key function for in planta fitness.
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影响因子：
4.4
作者：
Aury JM;Cruaud C;Barbe V;Rogier O;Mangenot S;Samson G;Poulain J;Anthouard V;Scarpelli C;Artiguenave F;Wincker P
通讯作者：
Wincker P
影响因子：
6.7
作者：
Baltrus DA;Nishimura MT;Romanchuk A;Chang JH;Mukhtar MS;Cherkis K;Roach J;Grant SR;Jones CD;Dangl JL
通讯作者：
Dangl JL
影响因子：
3.7
作者：
Blanvillain S;Meyer D;Boulanger A;Lautier M;Guynet C;Denancé N;Vasse J;Lauber E;Arlat M
通讯作者：
Arlat M
影响因子：
5.1
作者：
Bobrov, Alexander G.;Kirillina, Olga;Perry, Robert D.
通讯作者：
Perry, Robert D.
影响因子：
3.2
作者：
Alegria, MC;Souza, DP;Farah, CS
通讯作者：
Farah, CS