Transfer of a starch phenotype from wild wheat to bread wheat by deletion of a locus controlling B-type starch granule content.
Transfer of a starch phenotype from wild wheat to bread wheat by deletion of a locus controlling B-type starch granule content.
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DOI：
10.1093/jxb/erx349
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发表时间：
2017-11-28
影响因子：
6.9
通讯作者：
Trafford K
中科院分区：
文献类型：
--
作者：
Chia T;Adamski NM;Saccomanno B;Greenland A;Nash A;Uauy C;Trafford K
我们描述了面包小麦的生产和表征，其谷物缺乏小的B型淀粉颗粒，具有接近正常的重量和淀粉含量，并且具有新颖的谷物和淀粉物理化学性质。我们先前对四倍体野生小麦物种Aegilops peregrina的遗传分析预测，每个单倍体基因组中的单个基因Bgc-1控制着籽粒中的B型淀粉粒含量。为了研究面包小麦（Triticum aestivum L.）具有Bgc-1基因座的小麦群体，我们筛选了一个γ辐照面包小麦cv.跨Bgc-1的第4组染色体缺失的典范。在染色体4A和4D中发现了合适的缺失，每个缺失包含约600-700个基因。这两个缺失被预测有240个同源异形基因是共同的。与单缺失突变体相比，双缺失突变体缺乏B型淀粉粒。B-少籽粒具有正常的A型淀粉粒形态、正常的总淀粉含量和正常的粒重。除了淀粉颗粒大小分布的变化，B-少小麦籽粒不同，从控制在籽粒硬度，淀粉膨胀力，直链淀粉含量。我们认为，这些B-少小麦植物是唯一可用的小麦族谷物，其将淀粉颗粒尺寸分布的实质性改变与对淀粉含量的最小影响相结合。
We describe the production and characterization of bread wheat with grains that lack small B-type starch granules, have near-normal weight and starch content, and have novel grain and starch physicochemical properties. Our previous genetic analysis of a tetraploid wild wheat species, Aegilops peregrina, predicted that a single gene per haploid genome, Bgc-1, controls B-type starch granule content in the grain. To test whether bread wheat (Triticum aestivum L.) has orthologous Bgc-1 loci, we screened a population of γ-irradiated bread wheat cv. Paragon for deletions of the group 4 chromosomes spanning Bgc-1. Suitable deletions, each encompassing ~600–700 genes, were discovered for chromosomes 4A and 4D. These two deletions are predicted to have 240 homoeologous genes in common. In contrast to single deletion mutant plants, double deletion mutants were found to lack B-type starch granules. The B-less grains had normal A-type starch granule morphology, normal overall starch content, and normal grain weight. In addition to variation in starch granule size distribution, the B-less wheat grains differed from controls in grain hardness, starch swelling power, and amylose content. We believe that these B-less wheat plants are the only Triticeae cereals available that combine substantial alterations in starch granule size distribution with minimal impact on starch content.
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DOI：
10.1093/bioinformatics/btq033
发表时间：
2010-03-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Quinlan AR;Hall IM
通讯作者：
Hall IM
影响因子：
6.9
作者：
Howard T;Rejab NA;Griffiths S;Leigh F;Leverington-Waite M;Simmonds J;Uauy C;Trafford K
通讯作者：
Trafford K
影响因子：
2.4
作者：
Peng, M;Gao, M;Chibbar, RN
通讯作者：
Chibbar, RN
影响因子：
6.1
作者：
Li, Wenhao;Shan, Yulin;Zhang, Guoquan
通讯作者：
Zhang, Guoquan
影响因子：
3.8
作者：
PARKER, ML
通讯作者：
PARKER, ML