The role of inflammatory cytokines in anemia and gastrointestinal mucosal injury induced by foot electric stimulation.
The role of inflammatory cytokines in anemia and gastrointestinal mucosal injury induced by foot electric stimulation.
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炎症细胞因子在足部电刺激所致贫血及胃肠黏膜损伤中的作用
DOI：
10.1038/s41598-021-82604-7
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发表时间：
2021-02-04
影响因子：
4.6
通讯作者：
Cheng Y
中科院分区：
文献类型：
--
作者：
Fan F;Ai Y;Sun T;Li S;Liu H;Shi X;Zhang Z;Liu Q;Cheng Y
足部电刺激（FES）被认为是一种经典的应激源，可以扰乱稳态。FES致小鼠急性贫血。本研究的目的是探讨炎症细胞因子在FES急性贫血和胃肠道（GI）粘膜损伤中的作用。雄性昆明种小鼠24只，体重20 ± 2 g，随机分为对照组和实验组。将小鼠置于能产生0.5mA电脉冲的足电击室中，持续0.5h。分别检测红细胞计数、血红蛋白浓度、红细胞压积、血清和下丘脑促肾上腺皮质激素释放激素（CRH）、血清和垂体促肾上腺皮质激素（ACTH）水平。此外，我们还通过聚合酶链反应（PCR）检测了下丘脑和十二指肠中炎性细胞因子（IL-1、IL-6、TNF-α、iNOS和IL-10）的表达。结果表明，FES可引起大鼠贫血，血清CRH和ACTH活性升高。IL-1β、IL-6、TNF-α和iNOS的表达在此过程中显著增加，而IL-10未被激活。提示FES致小鼠贫血、下丘脑和十二指肠炎性细胞因子与胃肠道粘膜损伤/出血密切相关。总之，这些结果强调了贫血、GI粘膜损伤/出血和应激的重要性，需要进一步研究将这些发现转化为受影响患者的获益。
Foot electrical stimulation (FES) has been considered as a classic stressor that can disturb homeostasis. Acute anemia was observed in the model induced by FES. The aim of this study was to explore the role of inflammatory cytokines underlying the acute anemia and gastrointestinal (GI) mucosal injury in the FES. Twenty-four male Kunming mice (20 ± 2 g) were randomly divided into control group and experimental group. The mice were placed in a footshock chamber that can generate 0.5 mA electrical impulse periodically for 0.5 h. After the process, red blood cell count, hemoglobin concentration and hematocrit, the levels of corticotropin releasing hormone (CRH) in serum and hypothalamus, and adrenocorticotropic hormone (ACTH) in serum and pituitary were detected separately. In addition, we investigated the expressions of inflammatory cytokines (IL-1, IL-6, TNF-α, iNOS, and IL-10) in the hypothalamus and duodenum by Polymerase Chain Reaction (PCR). Results showed that this FES model induced anemia, increased CRH and ACTH activity in the serum after the FES. Moreover, the expressions of IL-1β, IL-6, TNF-α, and iNOS were significantly increased following the process, while IL-10 was not activated. These findings suggest that anemia, the inflammatory cytokines in the hypothalamus and duodenum of the mice in the model induced by FES is closely related to GI mucosal injury/bleeding. Taken together, these results underscore the importance of anemia, GI mucosal injury/bleeding and stress, future studies would be needed to translate these findings into the benefit of affected patients.
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影响因子：
6
作者：
Al-Sadi, Rana;Guo, Shuhong;Ma, Thomas Y.
通讯作者：
Ma, Thomas Y.
影响因子：
4.3
作者：
Bjornsson, Bergthor;Winbladh, Anders;Sandstrom, Per
通讯作者：
Sandstrom, Per
影响因子：
29.4
作者：
Atreya, Raja;Zimmer, Michael;Neurath, Markus F.
通讯作者：
Neurath, Markus F.
影响因子：
4.6
作者：
Barksby, H. E.;Lea, S. R.;Taylor, J. J.
通讯作者：
Taylor, J. J.
DOI：
10.1152/ajpgi.00324.2001
发表时间：
2002-08-01
影响因子：
4.5
作者：
Murray, IA;Daniels, I;Long, RG
通讯作者：
Long, RG