ASYMMETRIC LEAVES2-LIKE11 gene, a member of the AS2/LOB family of Arabidopsis, causes pleiotropic alteration in transgenic cockscomb (Celosia cristata)
ASYMMETRIC LEAVES2-LIKE11 gene, a member of the AS2/LOB family of Arabidopsis, causes pleiotropic alteration in transgenic cockscomb (Celosia cristata)
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ASYMMETRIC LEAVES2-LIKE11 基因是拟南芥 AS2/LOB 家族的成员，可引起转基因鸡冠花（鸡冠花）的多效性改变
DOI：
10.1007/s11240-010-9676-x
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发表时间：
2010-02
期刊：
影响因子：
--
通讯作者：
Jian Zhu.
中科院分区：
文献类型：
--
作者：
Xu-Dong Sun;Lai-Sheng Meng;Zhen-Hua Feng;Jian Zhu.
拟南芥ASYMMETRIC LEAVES 2-LIKE 11基因是拟南芥ASYMMETRIC LEAVES 2（AS 2）/LATERAL ORGAN BOUNDARIES（LOB）结构域基因家族的成员。除ASYMMETRIC LEAVES 2（as 2）外，目前已报道的AS 2/LOB家族基因突变体均未表现出明显的表型。因此，突变asl 11的影响很可能被功能冗余所掩盖。为了阐明ASL 11基因在植物生长发育中的作用，我们通过农杆菌介导法将35 S：ASL 11基因导入拟南芥。数据表明，观察到的表型可能是由35 S：ASL 11触发的KNOX基因的错误表达引起的。然而，这些表型可能是由于产生ASL 11 RNA的影响而产生的假象，这可能影响AS 2/LOB家族的未鉴定成员的表达，该家族包括42个成员和其他未知基因。鸡冠花转化系统，我们已经建立了方便使用该物种的基因操作和表达的研究。因此，在这里，我们扩展我们的研究，包括额外的转化体，即，我们报告转化ASL 11基因鸡冠花植物。通过聚合酶链反应（PCR）鉴定了总共46个T1 35 S：ASL 11植株，并通过ASL 11-GFP融合蛋白的测定证实。部分35 S：ASL 11植株（13/46）的叶片呈浅裂或褶皱状。部分35 S：ASL 11植株（18/46）的茎尖分生组织呈针状结构，缺乏器官原基。数据表明，这些畸形表型可能是由35 S：ASL 11引起的KNOX基因异位表达引发的。总之，所有的数据表明，ASL 11，一个转录因子，可能参与调控KNOX基因的表达在植物发育。
ASYMMETRIC LEAVES2-LIKE11 gene of Arabidopsis is a member of the ArabidopsisASYMMETRIC LEAVES2 (AS2)/LATERAL ORGAN BOUNDARIES (LOB) domain gene family. With the exception of ASYMMETRIC LEAVES2 (as2), mutants of AS2/LOB family genes reported so far have not displayed any obvious phenotypes. Therefore, the effects of mutant asl11 are most likely masked by functional redundancy. To elucidate the role of ASL11 gene in plant growth and development, we introduced 35S:ASL11 to Arabidopsis plants via Agrobacterium tumefaciens. Data suggest that the observed phenotypes might be caused by misexpression of KNOX genes triggered by 35S:ASL11. However, it is possible that these phenotypes might be generated by an artifact due to the effect of the production of ASL11 RNAs, which might affect expression of unidentified members of the AS2/LOB family, which includes 42 members and other unknown genes. The cockscomb transformation systems that we have established facilitate the use of this species for studies on gene manipulation and expression. Therefore, here, we extend our studies to include additional transformants; namely, we report transformation of ASL11 gene to cockscomb plants. A total of 46 T1 35S:ASL11 plants were identified by polymerase chain reaction (PCR), confirmed by assay of ASL11-GFP fusion proteins. The leaves of some 35S:ASL11 plants (13/46) showed lobed or rumpled patterns. Shoot apical meristems of some 35S:ASL11 plants (18/46) displayed pin-like structures that lacked organ primordial. Data suggest that these malformed phenotypes might be triggered by ectopic expression of KNOX genes caused by 35S:ASL11. In general, all data indicate that ASL11, a transcription factor, might be involved in regulating KNOX gene expression in plant development.
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DOI：
10.1105/tpc.106.042325
发表时间：
2007-02
期刊：
The Plant Cell Online
影响因子：
--
作者：
Y. Ueno;T. Ishikawa;Keiro Watanabe;Shinji Terakura;H. Iwakawa;K. Okada;C. Machida;Y. Machida
通讯作者：
Y. Ueno;T. Ishikawa;Keiro Watanabe;Shinji Terakura;H. Iwakawa;K. Okada;C. Machida;Y. Machida
影响因子：
56.9
作者：
Kobayashi, Y;Kaya, H;Araki, T
通讯作者：
Araki, T
影响因子：
5.1
作者：
Aida, Mitsuhiro;Tasaka, Masao
通讯作者：
Tasaka, Masao
影响因子：
2.6
作者：
Lai-Sheng Meng;Jiang-Ping Song;Shaoshuang Sun;Chong Wang
通讯作者：
Lai-Sheng Meng;Jiang-Ping Song;Shaoshuang Sun;Chong Wang
影响因子：
4.6
作者：
Erik Vollbrecht;Leonore Reiser;Sarah Hake
通讯作者：
Erik Vollbrecht;Leonore Reiser;Sarah Hake