The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite.
The genome sequence of Rickettsia felis identifies the first putative conjugative plasmid in an obligate intracellular parasite.
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立克的立克氏菌的基因组序列鉴定出强制性细胞内寄生虫中的第一个假定的共轭质粒。
DOI：
10.1371/journal.pbio.0030248
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发表时间：
2005-08
期刊：
影响因子：
9.8
通讯作者：
Raoult D
中科院分区：
文献类型：
--
作者：
Ogata H;Renesto P;Audic S;Robert C;Blanc G;Fournier PE;Parinello H;Claverie JM;Raoult D
我们对猫立克次体（一种与跳蚤相关的专性细胞内α-变形杆菌，可引起人类斑疹热）的基因组进行了测序。除了1,485,148 bp的环状染色体外，猫毛鼠还显示出在专性细胞内细菌中首次发现的推定的共轭质粒。该质粒以短（39263 bp）和长（62829 bp）的形式存在。在许多其他特征方面，猫鼠与先前测序的立克次体形成对比，包括许多转座酶，几个染色体毒素抗毒素基因，更多的斑点基因，以及大量含有锚蛋白和四肽基序的基因。发现了patatin和RickA的宿主入侵相关基因。从基因组分析中预测的几种表型进行了实验测试：观察了接合毛和交配，以及β-内酰胺酶活性，肌动蛋白聚合驱动的迁移性和溶血特性。我们的研究表明，全基因组测序是揭示新近培养的专性胞内细菌表型特征的最快方法。猫立克次体是一种专性细胞内细菌，生活在跳蚤中并引起人类斑疹热。它的基因组序列提供了第一个证据，证明这种细菌可以进行结合。
We sequenced the genome of Rickettsia felis, a flea-associated obligate intracellular α-proteobacterium causing spotted fever in humans. Besides a circular chromosome of 1,485,148 bp, R. felis exhibits the first putative conjugative plasmid identified among obligate intracellular bacteria. This plasmid is found in a short (39,263 bp) and a long (62,829 bp) form. R. felis contrasts with previously sequenced Rickettsia in terms of many other features, including a number of transposases, several chromosomal toxin–antitoxin genes, many more spoT genes, and a very large number of ankyrin- and tetratricopeptide-motif-containing genes. Host-invasion-related genes for patatin and RickA were found. Several phenotypes predicted from genome analysis were experimentally tested: conjugative pili and mating were observed, as well as β-lactamase activity, actin-polymerization-driven mobility, and hemolytic properties. Our study demonstrates that complete genome sequencing is the fastest approach to reveal phenotypic characters of recently cultured obligate intracellular bacteria. Rickettsia felis is an obligate intracellular bacterium that lives in fleas and causes spotted fever in humans. Its genome sequence provides the first evidence that such bacteria can undergo conjugation.
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DOI：
10.1128/cdli.10.2.221-228.2003
发表时间：
2003-03-01
期刊：
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
影响因子：
--
作者：
Fang, R;Raoult, D
通讯作者：
Raoult, D
影响因子：
3.2
作者：
Beranek, A;Zettl, M;Koraimann, G
通讯作者：
Koraimann, G
影响因子：
3.2
作者：
Connell, TD;Metzger, DJ;Folster, JP
通讯作者：
Folster, JP
影响因子：
3.1
作者：
Chung, S;Mondon, P;Kwon-Chung, KJ
通讯作者：
Kwon-Chung, KJ
影响因子：
5.6
作者：
Gough, J;Karplus, K;Chothia, C
通讯作者：
Chothia, C