Genome-wide analysis of the endoplasmic reticulum stress response during lignocellulase production in Neurospora crassa.
Genome-wide analysis of the endoplasmic reticulum stress response during lignocellulase production in Neurospora crassa.
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粗糙脉孢菌木质纤维素酶生产过程中内质网应激反应的全基因组分析
DOI：
10.1186/s13068-015-0248-5
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发表时间：
2015
影响因子：
6.3
通讯作者：
Ma Y
中科院分区：
文献类型：
--
作者：
Fan F;Ma G;Li J;Liu Q;Benz JP;Tian C;Ma Y
背景：木质素纤维素分解真菌细胞在木质素纤维素酶合成过程中受到内质网（ER）胁迫;然而，在全基因组范围内对这一整合过程的理解仍然很差。本文对粗糙脉孢菌（Neurosporacrassa（N.结果：共鉴定出766个基因为N. crassa内质网应激反应靶基因（ESRTs）。crassa在纤维素利用条件下。其中，223和186个基因的表达分别显示出对IRE-1和HAC-1的依赖性。共筛选出527个ESRT基因突变体，其中249个表现出ER胁迫敏感性，包括100个功能未知的基因。在N. crassa不影响纤维素对木质纤维素酶基因的转录诱导，但严重影响相应酶的分泌。对转录因子的研究发现了三个新的调节因子（RES-1，RES-2，RRG-2）参与木质纤维素酶的分泌。与野生型（WT）相比，Δres-1中的木质纤维素酶的产量增加了30%以上，而Δres-2和Δrrg-2中的分泌减少了近30%。三个TF突变体的转录谱表明，他们深深地参与了木质纤维素酶分泌和ER stress respons.Conclusions：在这里，我们确定了在模式纤维素分解真菌N.粗鲁。通过全基因组突变体筛选和分析，发现了数十个新基因参与了这一过程。本研究的结果将有助于菌株的改良，以促进木质纤维素酶和生物质为基础的化学品的生产。
Background:Lignocellulolytic fungal cells suffer endoplasmic reticulum (ER) stress during lignocellulase synthesis; however, an understanding of this integrated process on a genome-wide scale remains poor. Here, we undertook a systematic investigation of this process in Neurospora crassa (N. crassa) using transcriptomic analysis coupled with genetic screens.Results:A set of 766 genes was identified as the ER stress response targets (ESRTs) in N. crassa under cellulose utilization conditions. Among these, the expression of 223 and 186 genes showed dependence on IRE-1 and HAC-1, respectively. A total of 527 available mutants for ESRT genes were screened, 249 of which exhibited ER stress susceptibility, including 100 genes with unknown function. Disruption of ire-1 or hac-1 in N. crassa did not affect transcriptional induction of lignocellulase genes by cellulose but severely affected secretion of the corresponding enzymes. A global investigation of transcription factors (TFs) discovered three novel regulators (RES-1, RES-2, RRG-2) involved in lignocellulase secretion. Production of lignocellulases in Δres-1 increased by more than 30% in comparison to wild type (WT), while secretion decreased by nearly 30% in strains Δres-2 and Δrrg-2. Transcriptional profiling of the three TF mutants suggests they are deeply involved in lignocellulase secretion and ER stress response.Conclusions:Here, we determined the transcriptional scope of the ER stress response during lignocellulase synthesis in the model cellulolytic fungus N. crassa. Through genome-wide mutant screening and analysis, dozens of novel genes were discovered to be involved in the process. The findings of this work will be useful for strain improvement to facilitate lignocellulase and biomass-based chemical production.
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影响因子：
4.4
作者：
Carvalho ND;Jørgensen TR;Arentshorst M;Nitsche BM;van den Hondel CA;Archer DB;Ram AF
通讯作者：
Ram AF
影响因子：
3.6
作者：
Benz JP;Chau BH;Zheng D;Bauer S;Glass NL;Somerville CR
通讯作者：
Somerville CR
影响因子：
9.8
作者：
Dombroski, Beth A.;Nayak, Renuka R.;Spielman, Richard S.
通讯作者：
Spielman, Richard S.
影响因子：
3.6
作者：
Al-Sheikh, H;Watson, AJ;Archer, DB
通讯作者：
Archer, DB
DOI：
10.1016/j.bbamcr.2013.01.016
发表时间：
2013-11-01
影响因子：
5.1
作者：
Hollien, Julie
通讯作者：
Hollien, Julie