A comparative systems analysis of polysaccharide-elicited responses in Neurospora crassa reveals carbon source-specific cellular adaptations.
A comparative systems analysis of polysaccharide-elicited responses in Neurospora crassa reveals carbon source-specific cellular adaptations.
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DOI：
10.1111/mmi.12459
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发表时间：
2014-01
影响因子：
3.6
通讯作者：
Somerville CR
中科院分区：
文献类型：
--
作者：
Benz JP;Chau BH;Zheng D;Bauer S;Glass NL;Somerville CR
丝状真菌是植物细胞壁多糖分解的水解酶的强大生产者。然而，这些糖在复杂的细胞壁基质中如何被感知的核心问题在很大程度上仍然难以捉摸。为了解决这个问题，在一个系统的方式，我们进行了广泛的比较系统分析的模式丝状真菌粗糙脉孢菌响应三个主要的细胞壁多糖：果胶，半纤维素和纤维素。我们发现果胶反应在很大程度上独立于纤维素分解反应，与半纤维素有一些重叠，并且在其程度上令人惊讶地高，这表明真菌的优势不仅仅是碳源。我们的方法还使我们能够识别碳源特异性适应，例如纤维素上未折叠蛋白质反应的诱导，以及可能参与碳侦察的一组常见诱导的29个基因。此外，通过层次聚类，我们生成了一个共表达矩阵，用于发现参与多糖利用的新组分。这是例证的鉴定lat-1，我们证明编码的生理相关的阿拉伯糖转运在脉孢菌。这里提供的分析是了解复杂生物质的真菌降解过程的重要一步。
Filamentous fungi are powerful producers of hydrolytic enzymes for the deconstruction of plant cell wall polysaccharides. However, the central question of how these sugars are perceived in the context of the complex cell wall matrix remains largely elusive. To address this question in a systematic fashion we performed an extensive comparative systems analysis of how the model filamentous fungus Neurospora crassa responds to the three main cell wall polysaccharides: pectin, hemicellulose and cellulose. We found the pectic response to be largely independent of the cellulolytic one with some overlap to hemicellulose, and in its extent surprisingly high, suggesting advantages for the fungus beyond being a mere carbon source. Our approach furthermore allowed us to identify carbon source-specific adaptations, such as the induction of the unfolded protein response on cellulose, and a commonly induced set of 29 genes likely involved in carbon scouting. Moreover, by hierarchical clustering we generated a co-expression matrix useful for the discovery of new components involved in polysaccharide utilization. This is exemplified by the identification of lat-1, which we demonstrate to encode for the physiologically relevant arabinose transporter in Neurospora. The analyses presented here are an important step towards understanding fungal degradation processes of complex biomass.
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影响因子：
4.4
作者：
Couturier M;Navarro D;Olivé C;Chevret D;Haon M;Favel A;Lesage-Meessen L;Henrissat B;Coutinho PM;Berrin JG
通讯作者：
Berrin JG
影响因子：
4.1
作者：
Christgau, S;Kofod, LV;Kauppinen, S
通讯作者：
Kauppinen, S
影响因子：
6.3
作者：
de Souza WR;de Gouvea PF;Savoldi M;Malavazi I;de Souza Bernardes LA;Goldman MHS;de Vries RP;de Castro Oliveira JV;Goldman GH
通讯作者：
Goldman GH
影响因子：
4.4
作者：
Benoit I;Coutinho PM;Schols HA;Gerlach JP;Henrissat B;de Vries RP
通讯作者：
de Vries RP
影响因子：
14.9
作者：
Finn RD;Mistry J;Tate J;Coggill P;Heger A;Pollington JE;Gavin OL;Gunasekaran P;Ceric G;Forslund K;Holm L;Sonnhammer EL;Eddy SR;Bateman A
通讯作者：
Bateman A