Pancreatic lipase-related protein 2 digests fats in human milk and formula in concert with gastric lipase and carboxyl ester lipase.
Pancreatic lipase-related protein 2 digests fats in human milk and formula in concert with gastric lipase and carboxyl ester lipase.
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DOI：
10.1038/pr.2013.90
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发表时间：
2013-08
影响因子：
3.6
通讯作者：
Lowe, Mark E.
中科院分区：
文献类型：
--
作者：
Johnson, Karin;Ross, Leah;Miller, Rita;Xiao, Xunjun;Lowe, Mark E.
膳食脂肪在被吸收之前必须被消化成脂肪酸和单酰基甘油。在成人中，脂肪酶依赖性胰腺甘油三酯脂肪酶（PTL）对脂肪消化有重要作用。在新生的啮齿动物和人类中，胰腺表达低水平的PTL。在啮齿类动物中，PTL的同源物胰腺脂肪酶相关蛋白2 （PLRP2）和羧基酯脂肪酶（CEL）弥补了PTL的缺乏。在人类新生儿中，PLRP2在膳食脂肪消化中的作用尚不清楚。为了阐明人类PLRP2影响新生儿膳食脂肪消化的可能性，我们测定了PLRP2对母乳和婴儿配方奶粉的活性。纯化重组PLRP2、胃脂肪酶和CEL对人乳和配方奶粉中脂肪的活性分别用每种脂肪酶单独和联合标准pH-stat测定进行测定。添加到母乳中的磷脂酶刺激了脂肪的消化。PLRP2和CEL对人乳和配方奶有活性。胃脂肪酶预消化增加了PLRP2对这两种底物的活性。综上所述，CEL和PLRP2活性与配方奶具有协同作用。PLRP2可以消化人乳和配方奶中的脂肪。PLRP2与CEL和胃脂肪酶协同作用，在体外消化人乳中的脂肪。
Dietary fats must be digested into fatty acids and monoacylglycerols prior to absorption. In adults, colipase-dependent pancreatic triglyceride lipase (PTL) contributes significantly to fat digestion. In newborn rodents and humans, the pancreas expresses low levels of PTL. In rodents, a homologue of PTL, pancreatic lipase related protein 2 (PLRP2) and carboxyl ester lipase (CEL) compensate for the lack of PTL. In human newborns, the role for PLRP2 in dietary fat digestion is unclear. To clarify the potential of human PLRP2 to influence dietary fat digestion in newborns, we determined PLRP2 activity against human milk and infant formula. The activity of purified recombinant PLRP2, gastric lipase and CEL against fats in human milk and formula was measured with each lipase alone and in combination with a standard pH-stat assay. Colipase added to human milk stimulated fat digestion. PLRP2 and CEL had activity against human milk and formula. Pre-digestion with gastric lipase increased PLRP2 activity against both substrates. Together, CEL and PLRP2 activity was additive with formula and synergistic with human milk. PLRP2 can digest fats in human milk and formula. PLRP2 acts in concert with CEL and gastric lipase to digest fats in human milk in vitro.
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DOI：
10.1073/pnas.0914625107
发表时间：
2010-05-11
影响因子：
11.1
作者：
Hancock, Angela M.;Witonsky, David B.;Di Rienzo, Anna
通讯作者：
Di Rienzo, Anna
影响因子：
29.4
作者：
Carrière, F;Renou, C;Verger, R
通讯作者：
Verger, R
影响因子：
3.5
作者：
JENSEN, RG;FERRIS, AM;HENDERSON, RA
通讯作者：
HENDERSON, RA
影响因子：
3.6
作者：
Armand, M;Hamosh, M;Hamosh, P
通讯作者：
Hamosh, P
影响因子：
29.4
作者：
CARRIERE, F;BARROWMAN, JA;LAUGIER, R
通讯作者：
LAUGIER, R