Autoradiographic Determination of Regional Cerebral Blood Flow in the Immature Rat
Autoradiographic Determination of Regional Cerebral Blood Flow in the Immature Rat
复制标题
幼鼠局部脑血流放射自显影测定
作者：
David T Lyons;F. Vasta;R. Vannucci
摘要。方法：7日龄大鼠（15g体重）皮下注射碘-[14C]安替比林（5 μCi）。在一段可变时间后，将每只幼犬斩首并采集动脉血进行闪烁计数。大脑立即被取出，要么准备进行同位素计数，要么冷冻用于放射自显影。测定脑：血分配系数[0.944±0.006 ml/g（平均值±SEM）]。脑血流（CBF）和区域血流（RCBF）均根据菲克方程导出的公式计算。CBF为66±4 ml/100 g/min（平均值±SEM），介于报道的1日龄大鼠CBF和成年大鼠CBF之间。射线自显像具有足够的质量，允许至少11个结构的微密度读数。RCBF范围从皮层下白质20 ml/100 g/min到脑干71 ml/100 g/min。未成熟大鼠RCBF占成年大鼠RCBF的比例以脑干最大。以前认为不可行，这项技术提供了一种可靠和相对简单的方法来测量非常小的实验动物的CBF和RCBF。
ABSTRACT.: Seven-day-old rats (15 g body weight) were injected subcutaneously with iodo-[14C] antipyrine (5 μCi). After a variable period, each pup was decapitated and arterial blood collected for scintillation counting. Brains were immediately removed and either prepared for isotopic counting or frozen for autoradiography. The brain:blood partition coefficient was determined [0.944 ± 0.006 ml/g (mean ± SEM)]. Both cerebral blood flow (CBF) and regional CBF (RCBF) were calculated according to a formula derived from the Fick equation. CBF equaled 66 ± 4 ml/100 g/min (mean ± SEM), a value midway between reported 1-day-old rat CBF and adult rat CBF. Autoradiographs were of sufficient quality to permit microdensitimetric readings of a minimum of 11 structures. RCBF ranged from 20 ml/100 g/min in subcortical white matter to 71 ml/100 g/min in the brain stem. Immature rat RCBF, as a proportion of adult rat RCBF, was greatest in brain stem. Previously thought not feasible, this technique provides a reliable and relatively simple means of measuring both CBF and RCBF in the very small laboratory animal.
在双放射性核素放射自显影技术中使用 123I 和 14C 同时测量 LCBF 和 LCMRgl。
影响因子：
8.3
作者：
Lear,JL;Jones,SC;Greenberg,JH;Fedora,TJ;Reivich,M
通讯作者：
Reivich,M