Circulating tumour cells lacking cytokeratin in breast cancer: the importance of being mesenchymal.
Circulating tumour cells lacking cytokeratin in breast cancer: the importance of being mesenchymal.
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DOI：
10.1111/j.1582-4934.2011.01285.x
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发表时间：
2011-05
影响因子：
5.3
通讯作者：
Gazzaniga P
中科院分区：
文献类型：
--
作者：
Gradilone A;Raimondi C;Nicolazzo C;Petracca A;Gandini O;Vincenzi B;Naso G;Aglianò AM;Cortesi E;Gazzaniga P
循环肿瘤细胞（CTC）是转移性乳腺癌预后的独立预测因子。然而，在三分之一的患者中，循环肿瘤细胞无法通过常规方法检测到。该研究的目的是评估转移性乳腺癌患者中表达间充质标志物的循环肿瘤细胞的预后价值。我们从55例转移性乳腺癌患者的血液中分离出CTC。CTC的特征在于细胞角蛋白和上皮间质转化的标志物。在24个月的随访中，CTC中间充质标志物的获得与患者的预后相关。CTC上存在间充质标志物比单独表达细胞角蛋白更准确地预测预后不良。由于CTC经常丢失上皮抗原，因此靶向上皮抗原的测定可能会错过最具侵袭性的细胞群。因此，迫切需要改进检测方法以鉴定经历上皮间质转化程序的CTC。
Circulating tumour cells (CTCs) are independent predictor of prognosis in metastatic breast cancer. Nevertheless, in one third of patients, circulating tumour cells are undetected by conventional methods. Aim of the study was to assess the prognostic value of circulating tumour cells expressing mesenchymal markers in metastatic breast cancer patients. We isolated CTC from blood of 55 metastatic breast cancer patients. CTC were characterized for cytokeratins and markers of epithelial mesenchymal transition. The gain of mesenchymal markers in CTC was correlated to prognosis of patients in a follow-up of 24 months. The presence of mesenchymal markers on CTC more accurately predicted worse prognosis than the expression of cytokeratins alone. Because of the frequent loss of epithelial antigens by CTC, assays targeting epithelial antigens may miss the most invasive cell population. Thus, there is an urgent need to improve detection methods to identify CTC which undergone epithelial mesenchymal transition program.
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影响因子：
158.5
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