p38SJ, a novel DINGG protein protects neuronal cells from alcohol induced injury and death.
p38SJ, a novel DINGG protein protects neuronal cells from alcohol induced injury and death.
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P38SJ是一种新型的Dingg蛋白，可保护神经元细胞免受酒精诱导的损伤和死亡的影响。
DOI：
10.1002/jcp.21903
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发表时间：
2009-12
影响因子：
5.6
通讯作者：
Darbinian, Nune
中科院分区：
文献类型：
--
作者：
Amini, Shohreh;Merabova, Nana;Khalili, Kamel;Darbinian, Nune
乙醇诱导神经元细胞损伤和死亡，通过失调的几个信号转导事件，控制，部分地，通过激活MAPK/ERK 1/2和/或失活其相应的磷酸酶，PP 1。最近，我们已经纯化了一种新的蛋白质的大小为38 kDa，p38 SJ，从愈伤组织培养的贯叶连翘，这属于一个新兴的DINGG家族的蛋白质与磷酸盐结合活性。在这里，我们表明，用p38 SJ处理神经元细胞可以保护细胞免受暴露于乙醇引起的损伤。此外，用p38 SJ预处理神经元细胞减少了促凋亡蛋白Bax的水平和与凋亡相关的一些事件，如半胱天冬酶3裂解。此外，通过诱导应激，酒精可以提高活性氧（ROS）的产生，导致超氧化物歧化酶（SOD）活性降低。我们的结果表明，p38 SJ恢复乙醇处理的神经细胞中的SOD活性。这些观察结果提供了一种新的生物学工具，用于开发新的方法来预防乙醇诱导的神经元细胞死亡，并可能治疗与酒精滥用相关的神经系统疾病。
Ethanol induces neuronal cell injury and death by dysregulating several signaling events that are controlled, in part, by activation of MAPK/ERK1/2 and/or inactivation of its corresponding phosphatase, PP1. Recently, we have purified a novel protein of 38 kDa in size, p38SJ, from a callus culture of Hypericum perforatum, which belongs to an emerging DINGG family of proteins with phosphate binding activity. Here, we show that treatment of neuronal cells with p38SJ protects cells against injury induced by exposure to ethanol. Furthermore, pre-treatment of neuronal cells with p38SJ diminishes the level of the pro-apoptotic protein Bax and some events associated with apoptosis such as caspase 3 cleavage. In addition, by inducing stress, alcohol can elevate production of reactive oxygen species (ROS) that leads to a decrease in the activity of superoxide dismutase (SOD). Our results showed that p38SJ restores the activity of SOD in the ethanol treated neuronal cells. These observations provide a novel biological tool for developing new approaches for preventing neuronal cell death induced by ethanol and possibly treament of neurological disorders associated with alcohol abuse.
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影响因子：
3.4
作者：
Lee, Dae Y.;Lee, Inchul;Lee, Myung G.
通讯作者：
Lee, Myung G.
影响因子：
2.9
作者：
Antonio, Angeline M.;Druse, Mary J.
通讯作者：
Druse, Mary J.
影响因子：
2.5
作者：
Ku, Bo Mi;Lee, Yeon Kyung;Kang, Sang Soo
通讯作者：
Kang, Sang Soo
影响因子：
2.5
作者：
Heaton, MB;Paiva, M;Miller, R
通讯作者：
Miller, R
DOI：
10.1016/s0925-4439(01)00104-1
发表时间：
2002-04-24
影响因子：
6.2
作者：
Adams, L;Davey, S;Scott, K
通讯作者：
Scott, K