CTCF and ncRNA Regulate the Three-Dimensional Structure of Antigen Receptor Loci to Facilitate V(D)J Recombination.
CTCF and ncRNA Regulate the Three-Dimensional Structure of Antigen Receptor Loci to Facilitate V(D)J Recombination.
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DOI：
10.3389/fimmu.2014.00049
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发表时间：
2014
影响因子：
7.3
通讯作者：
Feeney AJ
中科院分区：
文献类型：
--
作者：
Choi NM;Feeney AJ
在免疫球蛋白重链和kappa轻链位点上，分布在> 2mb上的>100个功能变量(V)基因必须在3D空间中靠近(D)J基因，以创建多样化的抗体库。类似的事件发生在T细胞受体（TCR）位点，从而产生广泛的TCR库。在这篇综述中，我们将讨论CTCF在抗原受体（AgR）位点形成玫瑰状结构中的作用，以及它在促进和抑制V(D)J重排中交替发挥的各种作用。此外，非编码rna，也被称为种系转录，可以塑造Igh位点的三维结构，可能也可以塑造其他AgR位点的三维结构。在Igh基因座上，这可以通过将VH基因座上的转录区域聚集到Iμ转录的同一转录工厂中来实现。由于Iμ启动子Eμ与一个V基因最终重排到的DJH重排位点相邻，因此生殖系转录过程本身，特别是在VH位点的远端，可能在位点的压实中起重要而直接的作用。最后，我们将讨论Igh位点的转录和表观遗传景观对VH基因重排频率的影响。
At both the immunoglobulin heavy and kappa light chain loci, there are >100 functional variable (V) genes spread over >2 Mb that must move into close proximity in 3D space to the (D)J genes to create a diverse repertoire of antibodies. Similar events take place at the T cell receptor (TCR) loci to create a wide repertoire of TCRs. In this review, we will discuss the role of CTCF in forming rosette-like structures at the antigen receptor (AgR) loci, and the varied roles it plays in alternately facilitating and repressing V(D)J rearrangements. In addition, non-coding RNAs, also known as germline transcription, can shape the 3D configuration of the Igh locus, and presumably that of the other AgR loci. At the Igh locus, this could occur by gathering the regions being transcribed in the VH locus into the same transcription factory where Iμ is being transcribed. Since the Iμ promoter, Eμ, is adjacent to the DJH rearrangement to which one V gene will ultimately rearrange, the process of germline transcription itself, prominent in the distal half of the VH locus, may play an important and direct role in locus compaction. Finally, we will discuss the impact of the transcriptional and epigenetic landscape of the Igh locus on VH gene rearrangement frequencies.
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影响因子：
4.4
作者：
Degner, Stephanie C.;Wong, Timothy P.;Jankevicius, Gytis;Feeney, Ann J.
通讯作者：
Feeney, Ann J.
影响因子：
64.5
作者：
Guo C;Gerasimova T;Hao H;Ivanova I;Chakraborty T;Selimyan R;Oltz EM;Sen R
通讯作者：
Sen R
影响因子：
16
作者：
Hou, Chunhui;Li, Li;Qin, Zhaohui S.;Corces, Victor G.
通讯作者：
Corces, Victor G.
影响因子：
4.8
作者：
Featherstone, Karen;Wood, Andrew L.;Corcoran, Anne E.
通讯作者：
Corcoran, Anne E.
CTCF结合元件介导V（d）J重组的控制。
影响因子：
64.8
作者：
Guo, Chunguang;Yoon, Hye Suk;Franklin, Andrew;Jain, Suvi;Ebert, Anja;Cheng, Hwei-Ling;Hansen, Erica;Despo, Orion;Bossen, Claudia;Vettermann, Christian;Bates, Jamie G.;Richards, Nicholas;Myers, Darienne;Patel, Harin;Gallagher, Michael;Schlissel, Mark S.;Murre, Cornelis;Busslinger, Meinrad;Giallourakis, Cosmas C.;Alt, Frederick W.
通讯作者：
Alt, Frederick W.