Surface-Enhanced Raman Scattering Nanoparticles for Multiplexed Imaging of Bladder Cancer Tissue Permeability and Molecular Phenotype.
Surface-Enhanced Raman Scattering Nanoparticles for Multiplexed Imaging of Bladder Cancer Tissue Permeability and Molecular Phenotype.
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DOI：
10.1021/acsnano.8b03217
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发表时间：
2018-10-23
期刊：
影响因子：
17.1
通讯作者：
Gambhir SS
中科院分区：
文献类型：
--
作者：
Davis RM;Kiss B;Trivedi DR;Metzner TJ;Liao JC;Gambhir SS
膀胱癌的复发率是所有癌症中最高的，部分原因是经尿道切除术不充分。切除不充分是由于膀胱镜在切除过程中无法检测到不可见的病变。为了改善非肌肉浸润性膀胱癌的检测和切除，我们量化了表面增强拉曼纳米颗粒和内窥镜系统将膀胱组织分类为正常或癌性的能力。通过将纳米颗粒局部应用于离体人膀胱组织样品来评估基于抗体的（主动）和基于组织渗透性的（被动）靶向机制。CD47和碳酸酐酶9肿瘤蛋白的多重分子成像给出了受试者操作特征曲线下面积（ROC AUC为0.93（0.75，1.00）。此外，被动靶向纳米颗粒能够进行组织分类，ROC AUC为0.93（0.73，1.00）。与正常膀胱尿路上皮细胞相比，被动靶向纳米颗粒在癌症中渗透5倍更深，并以高3.3倍的浓度结合到肿瘤组织，表明在人类膀胱癌中存在增强的表面渗透性和保留效应。
Bladder cancer has the highest recurrence rate of all cancers due in part to inadequate transurethral resection. Inadequate resection is caused by the inability of cystoscopes to detect invisible lesions during the resection procedure. To improve detection and resection of nonmuscle invasive bladder cancer, we quantified the ability of a surface-enhanced Raman nanoparticle and endoscope system to classify bladder tissue as normal or cancerous. Both antibody-based (active) and tissue permeability-based (passive) targeting mechanisms were evaluated by topically applying nanoparticles to ex vivo human bladder tissue samples. Multiplexed molecular imaging of CD47 and Carbonic Anhydrase 9 tumor proteins gave a receiver operating characteristic area under the curve (ROC AUC of 0.93 (0.75, 1.00). Furthermore, passively targeted nanoparticles enabled tissue classification with an ROC AUC of 0.93 (0.73, 1.00). Passively targeted nanoparticles penetrated 5-fold deeper and bound to tumor tissue at 3.3-fold higher concentrations in cancer compared to normal bladder urothelium, suggesting the existence of an enhanced surface permeability and retention effect in human bladder cancer.
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影响因子：
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