A real-time clinical endoscopic system for intraluminal, multiplexed imaging of surface-enhanced Raman scattering nanoparticles.
A real-time clinical endoscopic system for intraluminal, multiplexed imaging of surface-enhanced Raman scattering nanoparticles.
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DOI：
10.1371/journal.pone.0123185
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Contag CH
中科院分区：
文献类型：
--
作者：
Garai E;Sensarn S;Zavaleta CL;Loewke NO;Rogalla S;Mandella MJ;Felt SA;Friedland S;Liu JT;Gambhir SS;Contag CH
人体胃肠道中生物标志物靶向表面增强拉曼散射（Sers）纳米颗粒（NP）的检测有可能改善早期癌症检测;然而，尚未描述具有快速拉曼成像能力的临床相关设备。在这里，我们报告的设计和体内演示的微型，非接触式，光电机械拉曼装置作为附件，临床内窥镜，可以提供多路复用的分子数据，通过一个面板的Sers纳米粒子。该装置能够快速圆周扫描中空器官的拓扑复杂的管腔表面（例如，结肠和食道），并产生存在的Sers NP的相对浓度的定量图像。人类和猪的研究已经证明了这种技术的速度和简单性。这种方法还通过同时检测多个Sers NP的独特光谱指纹提供了无与伦比的多路复用能力。因此，这种新的筛查策略有可能改善诊断和指导治疗，使敏感的定量分子检测的小，否则难以检测病变的背景下，白光内窥镜。
The detection of biomarker-targeting surface-enhanced Raman scattering (SERS) nanoparticles (NPs) in the human gastrointestinal tract has the potential to improve early cancer detection; however, a clinically relevant device with rapid Raman-imaging capability has not been described. Here we report the design and in vivo demonstration of a miniature, non-contact, opto-electro-mechanical Raman device as an accessory to clinical endoscopes that can provide multiplexed molecular data via a panel of SERS NPs. This device enables rapid circumferential scanning of topologically complex luminal surfaces of hollow organs (e.g., colon and esophagus) and produces quantitative images of the relative concentrations of SERS NPs that are present. Human and swine studies have demonstrated the speed and simplicity of this technique. This approach also offers unparalleled multiplexing capabilities by simultaneously detecting the unique spectral fingerprints of multiple SERS NPs. Therefore, this new screening strategy has the potential to improve diagnosis and to guide therapy by enabling sensitive quantitative molecular detection of small and otherwise hard-to-detect lesions in the context of white-light endoscopy.
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影响因子：
10.8
作者：
Zavaleta C;de la Zerda A;Liu Z;Keren S;Cheng Z;Schipper M;Chen X;Dai H;Gambhir SS
通讯作者：
Gambhir SS
影响因子：
9.3
作者：
Heresbach, D.;Barrioz, T.;Ponchan, T.
通讯作者：
Ponchan, T.
影响因子：
4.6
作者：
Dinish US;Balasundaram G;Chang YT;Olivo M
通讯作者：
Olivo M
DOI：
10.1073/pnas.0710575105
发表时间：
2008-04-15
影响因子：
11.1
作者：
Keren, S.;Zavaleta, C.;Gambhir, S. S.
通讯作者：
Gambhir, S. S.
影响因子：
120.7
作者：
Soetikno, Roy M.;Kaltenbach, Tonya;Friedland, Shai
通讯作者：
Friedland, Shai