Construction of Expanded Prefabricated Adipose Tissue Using an External Volume Expansion Device
Construction of Expanded Prefabricated Adipose Tissue Using an External Volume Expansion Device
复制标题
使用外部体积膨胀装置构建膨胀预制脂肪组织
DOI：
10.1097/prs.0000000000003277
复制
发表时间：
2017-01
影响因子：
3.6
通讯作者：
Lu Feng
中科院分区：
文献类型：
--
作者：
Yuan Yi;Yang Shuifa;Yi Yangyan;Gao Jianhua;Lu Feng
背景：多项研究表明，机械力促进脂肪组织的生长。然而，机械力诱导脂肪组织再生的机制仍不清楚。方法：在一项实验中，使用大鼠，预制脂肪组织血管蒂扩张使用外部体积扩张装置。在不同时间点测量脂肪瓣的体积。使用免疫荧光分析细胞增殖和血管生成。分别采用实时聚合酶链反应分析和酶联免疫吸附试验评价成脂基因和炎性细胞因子的表达。结果：实验组CD 31+细胞和Ki 67/CD 34+细胞明显高于对照组。Ki 67/CD 34+细胞数量在1 ~ 4周达到高峰。然而，过氧化物酶体增殖物激活受体和CCAAT/增强子结合蛋白的表达水平在实验组中从4周到12周最高。与对照组相比，实验组表达更多的促炎细胞因子：白细胞介素-1、白细胞介素-6、肿瘤坏死因子-α和巨噬细胞移动抑制因子。结论：利用机械力构建预构脂肪组织是一个动态的复杂过程。机械力在脂肪组织再生的早期（4周前）促进细胞增殖和血管生成，并在后期（4周后）通过上调巨噬细胞移动抑制因子诱导成脂分化，这提供了成脂诱导微环境。
Background: Multiple studies have demonstrated that mechanical forces promote the growth of adipose tissue. However, the mechanism of adipose tissue regeneration induced by mechanical forces remains unclear. Methods: In an experiment using rats, prefabricated adipose tissue with a vessel pedicle was expanded using an external volume expansion device. The volume of fat flaps was tested at different time points. Cell proliferation and angiogenesis were analyzed using immunofluorescence. The expression of adipogenic genes and inflammatory cytokines was evaluated using real-time polymerase chain reaction analysis and enzyme-linked immunosorbent assay, respectively. Results: There were more CD31+ cells and Ki67/CD34+ cells in the experimental group than in the control group. The number of Ki67/CD34+ cells peaked at 1 to 4 weeks. However, the expression levels of peroxisome proliferator-activated receptor-&ggr; and CCAAT/enhancer-binding protein-&bgr; were highest from 4 to 12 weeks in the experimental group. Compared with the control group, the experimental group showed more proinflammatory cytokines: interleukin-1&bgr;, interleukin-6, tumor necrosis factor-&agr;, and macrophage migration inhibitory factor. Conclusions: The construction of expanded prefabricated adipose tissue by mechanical forces is a dynamic and complex process. Mechanical forces promoted cell proliferation and angiogenesis in the early stage of adipose tissue regeneration (before 4 weeks) and induced adipogenic differentiation at a later stage (after 4 weeks) through up-regulation of macrophage migration inhibitory factor, which provided an adipogenic inductive microenvironment.
登录
查看更多内容
影响因子：
4.8
作者：
Ikeda, Daisuke;Sakaue, Shinji;Nishimura, Masaharu
通讯作者：
Nishimura, Masaharu
影响因子：
4.4
作者：
Atsumi, Toshiya;Cho, You-Ree;Bucala, Richard
通讯作者：
Bucala, Richard
影响因子：
3.6
作者：
R. Khouri;I. Schlenz;B. Murphy;T. J. Baker
通讯作者：
R. Khouri;I. Schlenz;B. Murphy;T. J. Baker
影响因子：
3.6
作者：
Heit, Yvonne I.;Lancerotto, Luca;Orgill, Dennis P.
通讯作者：
Orgill, Dennis P.
影响因子：
3.6
作者：
S. Slavin
通讯作者：
S. Slavin