Conjoint Analysis of Genome-Wide lncRNA and mRNA Expression of Heteromorphic Leavesin Response to Environmental Heterogeneityin Populus euphratica
Conjoint Analysis of Genome-Wide lncRNA and mRNA Expression of Heteromorphic Leavesin Response to Environmental Heterogeneityin Populus euphratica
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胡杨异形叶蛋白对环境异质性响应的全基因组 lncRNA 和 mRNA 表达联合分析
DOI：
10.3390/ijms20205148
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发表时间：
2019-10
影响因子：
5.6
通讯作者：
Yuanyuan Zhao
中科院分区：
文献类型：
--
作者：
Ming Zeng;Shuhang He;Lin Hao;Yujie Li;Caixia Zheng;Yuanyuan Zhao
异叶性是指一株植物的叶形沿纵轴变化的现象。胡杨是一种异叶木本植物，在树冠底部和顶部分别发育披针形叶片和具牙齿的宽卵形叶片，分别面对不同强度的环境太阳辐射。然而，胡杨异形叶片对微环境的响应机制仍然不清楚。在这里，我们表明，具牙齿的宽卵形叶片在耐高光强度方面具有优势，而披针形叶片在捕捉光线方面具有很好的优势。与披针形叶片相比，具牙齿的宽卵形叶片具有更多的毛状体、更高的气孔密度、更厚的叶片和更大的比叶重。此外，高通量RNA测序分析表明，两个异形叶片之间的基因和长非编码RNA(LncRNAs)的表达模式不同。共检测到36,492个基因和1725个lncRNAs，其中586个基因和54个lncRNAs存在差异表达。根据靶标预测，参与光适应、蛋白质修复、逆境响应和生长发育途径的lncRNAs和靶基因在异形叶片中差异表达，其中10对通过实时荧光定量PCR得到证实。此外，互作分析表明，lncRNA-mRNA的相互作用参与了异形叶片对微环境的响应。综上所述，这些结果表明，异形叶片的形态特征和lncRNA-mRNA的联合调控可能是胡杨的生存策略，从而可能导致环境因素的最佳利用。
Heterophylly is the phenomenon of leaf forms varying along the longitudinal axis within a single plant. Populus euphratica, a heterophyllous woody plant, develops lanceolate leaves and dentate broad-ovate leaves on the bottom and top of the canopy, respectively, which are faced with different intensities of ambient solar radiation. However, the mechanism of the heteromorphic leaf response to the microenvironment in P. euphratica remains elusive. Here, we show that the dentate broad-ovate leaves have advantages in tolerating high light intensity, while lanceolate leaves are excellent at capturing light. Compared with lanceolate leaves, more trichomes, higher stomatal density, thicker lamina, and higher specific leaf weight were observed in dentate broad-ovate leaves. Furthermore, high-throughput RNA sequencing analysis revealed that the expression patterns of genes and long noncoding RNAs (lncRNAs) are different between the two heteromorphic leaves. A total of 36,492 genes and 1725 lncRNAs were detected, among which 586 genes and 54 lncRNAs were differentially expressed. Based on targets prediction, lncRNAs and target genes involved in light adaption, protein repair, stress response, and growth and development pathways were differentially expressed in heteromorphic leaves, 10 pairs of which were confirmed by quantitative real-time PCR. Additionally, the analysis of interactions indicated that lncRNA–mRNA interactions were involved in the response to the microenvironment of heteromorphic leaves. Taken together, these results suggest that the morphological features and joint regulation of lncRNA–mRNA in heteromorphic leaves may serve as survival strategies for P. euphratica, which could lead to optimal utilization of environmental factors.
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