Macroscopic fluorescence imaging: a novel technique to monitor retention and distribution of injected microspheres in an experimental model of ischemic heart failure.
Macroscopic fluorescence imaging: a novel technique to monitor retention and distribution of injected microspheres in an experimental model of ischemic heart failure.
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DOI：
10.1371/journal.pone.0101775
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Kutschka I
中科院分区：
文献类型：
--
作者：
Martens A;Rojas SV;Baraki H;Rathert C;Schecker N;Hernandez SR;Schwanke K;Zweigerdt R;Martin U;Saito S;Haverich A;Kutschka I
心脏细胞疗法的有限有效性引起了对其临床相关性的关注。实验研究表明，注射到缺血心肌后，细胞滞留和植入率低，这可能会显着限制治疗效果。手术方面和机械损失被怀疑是这一现象背后的罪魁祸首。由于目前监测心肌内注射的技术复杂且耗时，本研究的目的是开发一种快速简单的模型来研究心肌内注射后的心脏保留和分布。为此，我们的主要假设是，宏观荧光成像可以充分作为心肌内注射的检测方法。共20只小鼠结扎左前降支（LAD）以造成心肌梗死。使用具有细胞尺寸的荧光微球作为细胞替代物。将5×105个微粒分别注入离体静息心脏（Exvivo myocardial injections EVMI，n = 10）和在体跳动心脏（Invivo myocardial injections IVMI，n = 10）的梗死区。 微球定量通过荧光成像的器官。在减少至匀浆稀释液后重复测量。EVMI组心脏微球滞留量为2.78×105±0.31×105。IVMI组微球的心脏滞留量显著低于对照组（0.74×105±0.18×105; p<0.05）。直接荧光成像显示通过冠状窦的静脉引流，导致左肺（0.90×105±0.20×105）和右肺（1.07×105±0.17×105）中的微球积聚。在两组中，均质物的处理涉及进一步的颗粒损失（p<0.05）。我们开发了一种快速，简单的直接荧光成像方法的生物分布分析，使定量的荧光微球后，心肌内交付使用宏观荧光成像。这项新技术显示大量早期颗粒丢失和静脉引流至右心房，导致移植物颗粒在双肺中大量积聚。
The limited effectiveness of cardiac cell therapy has generated concern regarding its clinical relevance. Experimental studies show that cell retention and engraftment are low after injection into ischemic myocardium, which may restrict therapy effectiveness significantly. Surgical aspects and mechanical loss are suspected to be the main culprits behind this phenomenon. As current techniques of monitoring intramyocardial injections are complex and time-consuming, the aim of the study was to develop a fast and simple model to study cardiac retention and distribution following intramyocardial injections. For this purpose, our main hypothesis was that macroscopic fluorescence imaging could adequately serve as a detection method for intramyocardial injections. A total of 20 mice underwent ligation of the left anterior descending artery (LAD) for myocardial infarction. Fluorescent microspheres with cellular dimensions were used as cell surrogates. Particles (5×105) were injected into the infarcted area of explanted resting hearts (Ex vivo myocardial injetions EVMI, n = 10) and in vivo into beating hearts (In vivo myocardial injections IVMI, n = 10). Microsphere quantification was performed by fluorescence imaging of explanted organs. Measurements were repeated after a reduction to homogenate dilutions. Cardiac microsphere retention was 2.78×105±0.31×105 in the EVMI group. In the IVMI group, cardiac retention of microspheres was significantly lower (0.74×105±0.18×105; p<0.05). Direct fluorescence imaging revealed venous drainage through the coronary sinus, resulting in a microsphere accumulation in the left (0.90×105±0.20×105) and the right (1.07×105±0.17×105) lung. Processing to homogenates involved further particle loss (p<0.05) in both groups. We developed a fast and simple direct fluorescence imaging method for biodistribution analysis which enabled the quantification of fluorescent microspheres after intramyocardial delivery using macroscopic fluorescence imaging. This new technique showed massive early particle loss and venous drainage into the right atrium leading to substantial accumulation of graft particles in both lungs.
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DOI：
10.1161/circinterventions.112.000422
发表时间：
2013-12
期刊：
Circulation. Cardiovascular interventions
影响因子：
--
作者：
Behfar A;Latere JP;Bartunek J;Homsy C;Daro D;Crespo-Diaz RJ;Stalboerger PG;Steenwinckel V;Seron A;Redfield MM;Terzic A
通讯作者：
Terzic A
影响因子：
24
作者：
Terrovitis, John;Lautamaeki, Riikka;Bonios, Michael;Fox, James;Engles, James M.;Yu, Jianhua;Leppo, Michelle K.;Pomper, Martin G.;Wahl, Richard L.;Seidel, Jurgen;Tsui, Benjamin M.;Bengel, Frank M.;Abraham, M. Roselle;Marban, Eduardo
通讯作者：
Marban, Eduardo
影响因子：
3.7
作者：
Clifford DM;Fisher SA;Brunskill SJ;Doree C;Mathur A;Clarke MJ;Watt SM;Martin-Rendon E
通讯作者：
Martin-Rendon E
DOI：
10.1016/j.jtcvs.2006.05.034
发表时间：
2006-09-01
影响因子：
6
作者：
Teng, Carolyn J.;Luo, Jun;Shum-Tim, Dominique
通讯作者：
Shum-Tim, Dominique
影响因子：
158.5
作者：
Schaechinger, Volker;Erbs, Sandra;Zeiher, Andreas M.
通讯作者：
Zeiher, Andreas M.