Inhibition by palmitoylcarnitine of adhesion and morphological changes in HL-60 cells induced by 12-O-tetradecanoylphorbol-13-acetate.
Inhibition by palmitoylcarnitine of adhesion and morphological changes in HL-60 cells induced by 12-O-tetradecanoylphorbol-13-acetate.
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棕榈酰肉碱对 12-O-十四烷酰佛波醇-13-乙酸酯诱导的 HL-60 细胞粘附和形态变化的抑制作用。
作者：
T. Nakaki;S. Mita;S. Yamamoto;T. Nakadate;R. Kato
研究了钙活化磷脂依赖性蛋白激酶（蛋白激酶C）抑制剂dl -棕榈酰基肉碱（PC）对12- o -十四烷醇-13-醋酸酯（TPA）诱导的人早幼粒细胞白血病细胞（HL-60）分化的影响。TPA引起HL-60细胞粘附，形态改变，酸性磷酸酶活性增加。中位有效浓度为1 nM，与[3H]TPA与细胞提取物的解离常数吻合。[3H]TPA与细胞提取物的结合是饱和可逆的。[3H] tpa结合位点的最大数目为1.5 pmol/mg蛋白，Hill系数为1，表明非合作相互作用。棕榈酸和dl -肉碱对tpa诱导的细胞粘附和形态变化均无抑制作用，中位抑制浓度为1 μ m，而3 μ m PC对tpa诱导的酸性磷酸酶活性的增加无影响。在添加TPA后1天或2天添加PC也能有效抑制细胞粘附。在各种酰基肉碱中，PC的作用最大。[3H]在一定浓度下，PC不抑制TPA与细胞提取物的结合，该浓度对TPA诱导的细胞粘附具有抑制作用。提示蛋白激酶C可能介导TPA诱导HL-60细胞分化。
Effects of DL-palmitoylcarnitine (PC), an inhibitor of calciumactivated, phospholipid-dependent protein kinase (protein kinase C), on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced cell differentiation were investigated in human promyelocytic leukemia cells (HL-60). TPA caused HL-60 cell adhesion concomitant with morphological changes, and an increase in acid phosphatase activity. The median effective concentration was 1 nM, which corresponded well to the dissociation constant of [3H]TPA binding to the cell extract. [3H]TPA binding to the cell extract was saturable and reversible. The maximal number of [3H]TPA-binding sites was 1.5 pmol/mg protein and a Hill coefficient was unity, indicating noncooperative interactions. PC, but neither palmitic acid nor DL-carnitine, inhibited the TPA-induced cell adhesion and morphological changes with the median inhibitory concentration of 1 microM, whereas a TPA-induced increase in acid phosphatase activity was not affected by 3 microM PC. Addition of PC 1 or 2 days after the addition of TPA was also effective in inhibiting the cell adhesion. Among various acylcarnitines, PC had the largest effect. [3H]TPA binding to the cell extract was not inhibited by PC at the concentration which was effective in inhibiting the TPA-induced cell adhesion. These results indicate that protein kinase C possibly mediates HL-60 cell differentiation induced by TPA.
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影响因子：
20.3
作者：
Cassileth,PA;Suholet,D;Cooper,RA
通讯作者：
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影响因子：
5.8
作者：
Wise,BC;Kuo,JF
通讯作者：
Kuo,JF
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影响因子：
20.3
作者：
Perussia,B;Lebman,D;Ip,SH;Rovera,G;Trinchieri,G
通讯作者：
Trinchieri,G
DOI：
10.1016/0006-291x(81)91779-4
发表时间：
1981-01-01
影响因子：
3.1
作者：
ASHENDEL, CL;BOUTWELL, RK
通讯作者：
BOUTWELL, RK
来自心脏的磷脂敏感 Ca2 依赖性蛋白激酶。
DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Wise,BC;Glass,DB;Chou,CH;Raynor,RL;Katoh,N;Schatzman,RC;Turner,RS;Kibler,RF;Kuo,JF
通讯作者：
Kuo,JF