No effect of natural transformation on the evolution of resistance to bacteriophages in the Acinetobacter baylyi model system.
No effect of natural transformation on the evolution of resistance to bacteriophages in the Acinetobacter baylyi model system.
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DOI：
10.1038/srep37144
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发表时间：
2016-11-21
影响因子：
4.6
通讯作者：
Vos M
中科院分区：
文献类型：
--
作者：
McLeman A;Sierocinski P;Hesse E;Buckling A;Perron G;Hülter N;Johnsen PJ;Vos M
自然转化的适应性益处，即从环境中主动摄取游离DNA分子，然后将该DNA掺入基因组中，可能是对遗传变异增加导致的选择的改善反应。与有性繁殖相类比，当选择在与有毒寄生虫的共同进化过程中迅速波动时，转化可能特别有益（“红皇后假说”）。在这里，我们测试这个假设的实验进化的自然转化和重组的物种不动杆菌baylyi与裂解酶的鸡尾酒。与重组缺陷型ΔdprA菌株相比，在进化5天后，野生型中未发现对噬菌体的抗性水平增加。当暴露于A.贝氏DNA和噬菌体，天然可转化细胞显示出更高水平的噬菌体抗性。然而，无论它们是否暴露于来自噬菌体敏感的或抗噬菌体的A的DNA，都产生了增加的抗性。baylyi，表明抗性不是转化的结果，而是与感受态的其他益处有关。随后的进化在没有重组的情况下并没有表明重组可以减轻抗性的代价。在这个研究系统中，我们发现没有支持转化介导的重组是暴露于寄生虫的细菌的优势。
The adaptive benefits of natural transformation, the active uptake of free DNA molecules from the environment followed by incorporation of this DNA into the genome, may be the improved response to selection resulting from increased genetic variation. Drawing analogies with sexual reproduction, transformation may be particularly beneficial when selection rapidly fluctuates during coevolution with virulent parasites (‘the Red Queen Hypothesis’). Here we test this hypothesis by experimentally evolving the naturally transformable and recombinogenic species Acinetobacter baylyi with a cocktail of lytic phages. No increased levels of resistance to phage were found in the wild type compared to a recombination deficient ΔdprA strain after five days of evolution. When exposed to A. baylyi DNA and phage, naturally transformable cells show greater levels of phage resistance. However, increased resistance arose regardless of whether they were exposed to DNA from phage-sensitive or –resistant A. baylyi, suggesting resistance was not the result of transformation, but was related to other benefits of competence. Subsequent evolution in the absence of phages did not show that recombination could alleviate the cost of resistance. Within this study system we found no support for transformation-mediated recombination being an advantage to bacteria exposed to parasitic phages.
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DOI：
10.1073/pnas.1315278110
发表时间：
2013-12-03
影响因子：
11.1
作者：
Overballe-Petersen, Soren;Harms, Klaus;Willerslev, Eske
通讯作者：
Willerslev, Eske
影响因子：
3.5
作者：
Murphy, KC;Campellone, KG;Poteete, AR
通讯作者：
Poteete, AR
DOI：
10.1098/rspb.2011.1933
发表时间：
2012-04-22
影响因子：
4.7
作者：
Perron, Gabriel G.;Lee, Alexander E. G.;Barraclough, Timothy G.
通讯作者：
Barraclough, Timothy G.
影响因子：
6.7
作者：
Engelmoer DJ;Donaldson I;Rozen DE
通讯作者：
Rozen DE
影响因子：
3.3
作者：
Baltrus, David A.;Guillemin, Karen;Phillips, Patrick C.
通讯作者：
Phillips, Patrick C.