Analysis of cell wall proteins regulated in stem of susceptible and resistant tomato species after inoculation with Ralstonia solanacearum: a proteomic approach.
Analysis of cell wall proteins regulated in stem of susceptible and resistant tomato species after inoculation with Ralstonia solanacearum: a proteomic approach.
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DOI：
10.1007/s11103-010-9646-z
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发表时间：
2010-08
影响因子：
5.1
通讯作者：
Wydra, Kerstin
中科院分区：
文献类型：
--
作者：
Dahal, Diwakar;Pich, Andreas;Braun, Hans Peter;Wydra, Kerstin
关键词：
利用蛋白质组学方法研究了青枯病菌（Ralstonia solanacearum）对番茄干细胞壁水平的影响。用盐缓冲液从纯化的细胞壁中提取抗病和感病植株接种细菌前后的细胞壁蛋白，并用2-D IEF/SDS-PAGE和3-D IEF/SDS/SDS-PAGE分离碱性蛋白。胶体考马斯染色的凝胶显示两个物种之间不同丰度的蛋白质点（8种蛋白质在较高丰度的抗性和其他6个敏感）。此外，蛋白质差异调节细菌接种在抗性（7个蛋白质增加，其他8个减少），以及在易感植物（5个蛋白质升高，其他8个抑制）。MALDI-TOF/TOF MS和LC-ESI-IonTrap MS/MS的组合导致这些蛋白质的鉴定。两种植物对病原菌的反应都是通过提高致病相关蛋白、其他防御相关蛋白和糖酵解蛋白的表达来实现的。而在感病株系中，细胞壁代谢蛋白被抑制，在抗病株系中，抗氧化、胁迫相关蛋白以及能量代谢蛋白被抑制。除了一些经典的胞质蛋白外，预测比较分析的大多数蛋白和其他随机挑选的点具有分泌信号。
Proteomics approach was used to elucidate the molecular interactions taking place at the stem cell wall level when tomato species were inoculated with Ralstonia solanacearum, a causative agent of bacterial wilt. Cell wall proteins from both resistant and susceptible plants before and after the bacterial inoculation were extracted from purified cell wall with salt buffers and separated with 2-D IEF/SDS–PAGE and with 3-D IEF/SDS/SDS–PAGE for basic proteins. The gels stained with colloidal Coomassie revealed varied abundance of protein spots between two species (eight proteins in higher abundance in resistant and six other in susceptible). Moreover, proteins were regulated differentially in response to bacterial inoculation in resistant (seven proteins increased and eight other decreased) as well as in susceptible plants (five proteins elevated and eight other suppressed). Combination of MALDI-TOF/TOF MS and LC-ESI-IonTrap MS/MS lead to the identification of those proteins. Plants responded to pathogen inoculation by elevating the expression of pathogenesis related, other defense related and glycolytic proteins in both species. However, cell wall metabolic proteins in susceptible, and antioxidant, stress related as well as energy metabolism proteins in resistant lines were suppressed. Most of the proteins of the comparative analysis and other randomly picked spots were predicted to have secretion signals except some classical cytosolic proteins.
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影响因子：
7.4
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
Wydra, Kerstin