Effects of low-dose embryonic thyroid disruption and rearing temperature on the development of the eye and retina in zebrafish.
Effects of low-dose embryonic thyroid disruption and rearing temperature on the development of the eye and retina in zebrafish.
复制标题
低剂量胚胎甲状腺破坏和饲养温度对斑马鱼眼睛和视网膜发育的影响。
DOI：
10.1002/bdrb.21118
复制
发表时间：
2014
期刊：
影响因子：
--
通讯作者：
V. Connaughton
中科院分区：
文献类型：
--
作者：
M. Reider;V. Connaughton
甲状腺激素是脊椎动物发育所必需的，发育胚胎中甲状腺系统的破坏可导致大范围的形态和生理变化，包括眼睛和视网膜。在这项研究中，我们的解剖分析后，低剂量，慢性甲状腺抑制显示，无论是他巴唑（MMI）曝光和饲养温度影响眼睛的发展，在时间和温度依赖性的方式。对于在28°C下饲养的斑马鱼，外眼发育对MMI的最大敏感性出现在受精后65 h（hpf），而对于在31°C下饲养的斑马鱼，则出现在受精后69 h。眼睛直径的变化对应于两个视网膜内层厚度的变化：神经节细胞层和内网状层，在28°C下用MMI处理的幼虫中观察到MMI诱导的不可逆层厚度降低，直至66 hpf。我们推断，在28°C下，在65和66 hpf之间对MMI的最大敏感性表明甲状腺依赖性眼睛和视网膜发育的关键时期。此外，我们的研究结果支持以前的工作，表明自发逃避MMI诱导的影响可能是由于胚胎代偿行动，因为我们的数据表明，胚胎治疗超过关键期一般类似于控制。
Thyroid hormones are required for vertebrate development, and disruption of the thyroid system in developing embryos can result in a large range of morphologic and physiologic changes, including in the eye and retina. In this study, our anatomic analyses following low-dose, chronic thyroid inhibition reveal that both methimazole (MMI) exposure and rearing temperature affect eye development in a time- and temperature-dependent fashion. Maximal sensitivity to MMI for external eye development occurred at 65 hr postfertilization (hpf) for zebrafish reared at 28°C, and at 69 hpf for those reared at 31°C. Changes in eye diameter corresponded to changes in thickness of two inner retinal layers: the ganglion cell layer and the inner plexiform layer, with irreversible MMI-induced decreases in layer thickness observed in larvae treated with MMI until 66 hpf at 28°C. We infer that maximal sensitivity to MMI between 65 and 66 hpf at 28°C indicates a critical period of thyroid-dependent eye and retinal development. Furthermore, our results support previous work that shows spontaneous escape from MMI-induced effects potentially due to embryonic compensatory actions, as our data show that embryos treated beyond the critical period generally resemble controls.
影响因子：
3.4
作者：
Zoeller Rt;K. Crofton
通讯作者：
Zoeller Rt;K. Crofton