Genomic and metabolic analysis of fluoranthene degradation pathway in Celeribacter indicus P73T.
Genomic and metabolic analysis of fluoranthene degradation pathway in Celeribacter indicus P73T.
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印度芹杆菌 P73T 荧蒽降解途径的基因组和代谢分析
DOI：
10.1038/srep07741
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发表时间：
2015-01-13
影响因子：
4.6
通讯作者：
Shao Z
中科院分区：
文献类型：
--
作者：
Cao J;Lai Q;Yuan J;Shao Z
从印度洋深海沉积物中分离到一株能降解多种多环芳烃（PAHs）的细菌，是杜鹃花科中第一株降解荧蒽的细菌。在这里，完整的基因组序列的菌株P73 T的介绍和分析。P73 T菌株除了一条4.5Mb的环状染色体外，还携带5个质粒，编码4827个预测的蛋白质编码序列。预计有138个基因（包括14个双加氧酶基因）参与芳香族化合物的降解，其中大多数基因聚集在四个区域。P73_0346是第一个被发现的荧蒽7，8-双加氧酶，也是甲苯/联苯家族中的第一个荧蒽双加氧酶。P73 T的B和D区的降解基因在白登藻B30中缺失，不能降解多环芳烃。P73 T降解荧蒽的主要中间产物为苊烯-1（2 H）-酮、苊醌、1，2-二羟基苊烯和1，8-萘二甲酸酐，表明P73 T降解荧蒽的起始反应是在C-7，8位双加氧。基于基因组和代谢的结果，我们提出了一个C-7，8双加氧途径，其中荧蒽被矿化为TCA循环中间体。
Celeribacter indicus P73T, isolated from deep-sea sediment from the Indian Ocean, is capable of degrading a wide range of polycyclic aromatic hydrocarbons (PAHs) and is the first fluoranthene-degrading bacterium within the family Rhodobacteraceae. Here, the complete genome sequence of strain P73T is presented and analyzed. Besides a 4.5-Mb circular chromosome, strain P73T carries five plasmids and encodes 4827 predicted protein-coding sequences. One hundred and thirty-eight genes, including 14 dioxygenase genes, were predicted to be involved in the degradation of aromatic compounds and most of these genes are clustered in four regions. P73_0346 is the first fluoranthene 7,8-dioxygenase to be discovered and the first fluoranthene dioxygenase within the toluene/biphenyl family. The degradative genes in regions B and D in P73T are absent in Celeribacterbaekdonensis B30, which cannot degrade PAHs. Four intermediate metabolites [acenaphthylene-1(2H)-one, acenaphthenequinone, 1,2-dihydroxyacenaphthylene and 1,8-naphthalic anhydride] of fluoranthene degradation by strain P73T were detected as the main intermediates, indicating that the degradation of fluoranthene in P73T was initiated by dioxygenation at the C-7,8 positions. Based on the genomic and metabolitic results, we propose a C-7,8 dioxygenation pathway in which fluoranthene is mineralized to TCA cycle intermediates.
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影响因子：
2.8
作者：
Keck, Andreas;Conradt, Doris;Klein, Joachim
通讯作者：
Klein, Joachim
影响因子：
3.7
作者：
Fukao M;Oshima K;Morita H;Toh H;Suda W;Kim SW;Suzuki S;Yakabe T;Hattori M;Yajima N
通讯作者：
Yajima N
影响因子：
14.9
作者：
Himmelreich, R;Hilbert, H;Herrmann, R
通讯作者：
Herrmann, R
影响因子：
3.2
作者：
Arias-Barrau, E;Olivera, ER;Miñambres, B
通讯作者：
Miñambres, B
影响因子：
5.2
作者：
Hickey WJ;Chen S;Zhao J
通讯作者：
Zhao J