IPB7 transposase behavior in Drosophila melanogaster and Aedes aegypti.
IPB7 transposase behavior in Drosophila melanogaster and Aedes aegypti.
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IPB7 转座酶在果蝇和埃及伊蚊中的行为。
DOI：
10.1016/j.ibmb.2013.06.009
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发表时间：
2013
影响因子：
3.8
通讯作者：
Atkinson,PeterW
中科院分区：
文献类型：
--
作者：
Wright,JenniferA;Smith,RyanC;Li,Xianghong;Craig,NancyL;Atkinson,PeterW
Transposons are used in insect science as genetic tools that enable the transformation of insects and the identification and isolation of genes though their ability to insert in or near to them. Four transposons,piggyBac,Mos1,HermesandMinosare commonly used in insects beyondDrosophila melanogasterwithpiggyBac, due to its wide host range and frequency of transposition, being the most commonly chosen. The utility of these transposons as genetic tools is directly proportional to their activity since higher transposition rates would be expected to lead to higher transformation frequencies and higher frequencies of insertion throughout the genome. As a consequence there is an ongoing need for hyperactive transposases for use in insect genetics, however these have proven difficult to obtain.IPB7is a hyperactive mutant of thepiggyBactransposase that was identified by a genetic screen performed in yeast, a mammalian codon optimized version of which was then found to be highly active in rodent embryonic stem cells with no apparent deleterious effects. Here we report the activity ofIPB7inD. melanogasterand the mosquito,Aedes aegypti. Somatic transposition assays revealed an increase inIPB7's transposition rate from wild-typepiggyBactransposase inD. melanogasterbut notAe. aegypti. However the use ofIPB7inD. melanogastergenetic transformations produced a high rate of sterility and a low transformation rate compared to wild-type transposase. This high rate of sterility was accompanied by significant gonadal atrophy that was also observed in the absence of thepiggyBacvector transposon. We conclude thatIPB7has increased activity in theD. melanogastergerm-line but that a component of the sterility associated with its activity is independent of the presence of thepiggyBactransposon.
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影响因子：
4.2
作者：
Doherty, Joseph E.;Huye, Leslie E.;Wilson, Matthew H.
通讯作者：
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影响因子：
64.5
作者：
BINGHAM, PM;KIDWELL, MG;RUBIN, GM
通讯作者：
RUBIN, GM
影响因子：
2.6
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Grossman, GL;Rafferty, CS;Benedict, MQ
通讯作者：
Benedict, MQ
影响因子：
4.8
作者：
Nolan, T;Bower, TM;Catteruccia, F
通讯作者：
Catteruccia, F
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作者：
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Ivics, Zoltan