Tomato LysM Receptor-Like Kinase SlLYK12 Is Involved in Arbuscular Mycorrhizal Symbiosis.
Tomato LysM Receptor-Like Kinase SlLYK12 Is Involved in Arbuscular Mycorrhizal Symbiosis.
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番茄 LysM 受体样激酶 SlLYK12 参与丛枝菌根共生
DOI：
10.3389/fpls.2018.01004
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发表时间：
2018
影响因子：
5.6
通讯作者：
Liang Y
中科院分区：
文献类型：
--
作者：
Liao D;Sun X;Wang N;Song F;Liang Y
丛枝菌根(AM)是植物和真菌(球菌门)之间广泛存在的一种共生关系，它能改善寄主植物的水分和营养供应。AM共生是由真菌壳寡糖和脂壳寡糖启动的，它们是由植物特异性的LysM型受体激酶(Lyk)感知的。在大米中，这与OsCERK1有关，这是一种赖克，也是甲壳素触发先天免疫所必需的。相反，在豆科植物中，CERK1同源基因经历了重复事件，导致了亚功能化。然而，目前尚不清楚这种亚功能化是豆科植物特有的，还是在其他双子叶植物物种中也存在。我们在番茄中鉴定了4个CERK1同源物(SlLYK1、SlLYK11、SlLYK12和SlLYK13)，并研究了它们在几丁质信号转导和AM共生中的作用。我们发现，SLLYK12基因在番茄中的敲除显著减少了AM的定植，而甲壳素诱导的反应不受影响。相反，SLLYK1基因敲除导致对甲壳素的反应减少，但不改变对AM真菌的反应。此外，异位过表达SlLYK1和SlLYK13在本氏烟草中诱导细胞死亡，而过表达SlLYK12不诱导细胞死亡。根据我们的结果以及与水稻OsCERK1的比较，我们推测番茄中的OsCERK1同源基因经历了基因复制，导致了免疫和共生的亚功能。
Arbuscular mycorrhiza (AM) is a widespread symbiotic relationship between plants and fungi (Glomeromycota), which improves the supply of water and nutrients to host plants. AM symbiosis is set in motion by fungal chitooligosaccharides and lipochitooligosaccharides, which are perceived by plant-specific LysM-type receptor kinases (LYK). In rice this involves OsCERK1, a LYK also essential for chitin triggered innate immunity. In contrast in legumes, the CERK1 homologous gene experienced duplication events resulting in subfunctionalization. However, it remains unknown whether this subfunctionalization is legume-specific, or has occurred also in other dicot plant species. We identified four CERK1 homologs in tomato (SlLYK1, SlLYK11, SlLYK12, and SlLYK13) and investigated their roles in chitin signaling and AM symbiosis. We found that knockdown of SlLYK12 in tomato significantly reduced AM colonization, whereas chitin-induced responses were unaffected. In contrast, knockdown of SlLYK1 resulted in reduced responses to chitin, but did not alter responses to AM fungi. Moreover, ectopic overexpression of SlLYK1 and SlLYK13 in Nicotiana benthamiana induced cell death, whereas SlLYK12 overexpression did not. Based on our results and comparison with rice OsCERK1, we hypothesize that OsCERK1 orthologs in tomato underwent gene duplication, leading to the subfunctionalization of immunity and symbiosis.
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影响因子：
7.2
作者：
Liu, Jinyuan;Maldonado-Mendoza, Ignacio;Harrison, Maria J.
通讯作者：
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影响因子：
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影响因子：
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作者：
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通讯作者：
Geurts, Rene
影响因子：
5.6
作者：
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通讯作者：
Dolgikh EA