Expression of EZH2 is associated with poor outcome in colorectal cancer.
Expression of EZH2 is associated with poor outcome in colorectal cancer.
复制标题
EZH2的表达与结直肠癌的结果不佳有关。
DOI：
10.3892/ol.2017.7647
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发表时间：
2018-03
期刊：
影响因子：
2.9
通讯作者：
Cao J
中科院分区：
文献类型：
--
作者：
Chen Z;Yang P;Li W;He F;Wei J;Zhang T;Zhong J;Chen H;Cao J
ZAST同源增强子2(EZH2)是多梳蛋白家族的重要组成部分，已被证明在多种人类肿瘤中过表达，包括肝细胞癌、乳腺癌、膀胱癌和肺癌。EZH2促进肿瘤发生的机制也得到了很好的研究。然而，对EZH2在结直肠癌(CRC)中的作用知之甚少。本研究的主要目的是分析EZH2的表达与结直肠癌临床病理特征的关系。因此，采用逆转录-定量聚合酶链式反应(RT-qPCR)和免疫印迹方法分析肿瘤组织和癌旁组织中的mRNA和蛋白表达水平。免疫印迹分析和RT-qPCR结果显示，EZH2在肿瘤组织中的表达明显高于癌旁组织。与EZH2高表达患者相比，EZH2低表达患者的总生存率更长。临床病理特征与EZH2表达的相关性分析表明，EZH2的高表达与肿瘤分期、肿瘤大小、组织分化程度及有无淋巴结转移有关。多因素分析表明，EZH2高表达是总生存期的独立预测因子。总而言之，就我们所知，本研究中的数据首次表明EZH2在结直肠癌中上调，并可能作为结直肠癌患者预后不良的预测因子。
Enhancer of zeste homolog 2 (EZH2), the critical component of polycomb group protein family, has been demonstrated to be overexpressed in various types of human cancer, including hepatocellular carcinoma, breast, bladder and lung cancer. The mechanism of how EZH2 promotes oncogenesis has also been well studied. However, little is known about the role of EZH2 in colorectal cancer (CRC). The main purpose of the present study was to analyze the association between EZH2 expression and the clinicopathological features of CRC. Therefore, the mRNA and protein expression levels were analyzed in tumor tissues and adjacent non-cancerous tissues by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blot analysis. The expression of EZH2 was demonstrated to be significantly increased in tumor tissues compared with adjacent noncancerous tissues, according to the results of western blot analysis and RT-qPCR in the majority of cases. Patients with low EZH2 expression had a longer overall survival rate compared with those with high EZH2 expression. An analysis of the association between clinicopathological features and EZH2 expression indicated that high EZH2 expression was significantly associated with tumor stage, tumor size, histological differentiation and lymph node metastasis. Multivariate analysis demonstrated that high EZH2 expression was an independent predictor of overall survival. In conclusion, to the best of our knowledge, the data presented in the present study is the first to indicate that EZH2 is upregulated in CRC and may serve as a predictor of poor outcome for patients with CRC.
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影响因子：
50.3
作者：
Chang CJ;Yang JY;Xia W;Chen CT;Xie X;Chao CH;Woodward WA;Hsu JM;Hortobagyi GN;Hung MC
通讯作者：
Hung MC
组蛋白去甲基化酶 KDM2B 上调组蛋白甲基转移酶 EZH2 表达并促进卵巢癌的体外和体内进展
影响因子：
4
作者：
Kuang Y;Lu F;Guo J;Xu H;Wang Q;Xu C;Zeng L;Yi S
通讯作者：
Yi S
影响因子：
2.9
作者：
Ma, Wenqi;Yu, Qiang;Zhou, Qi
通讯作者：
Zhou, Qi
影响因子：
64.8
作者：
Varambally, S;Dhanasekaran, SM;Chinnaiyan, AM
通讯作者：
Chinnaiyan, AM
影响因子：
6.2
作者：
Wittekind, C;Compton, CC;Sobin, LH
通讯作者：
Sobin, LH