The connexin43-dependent transcriptome during brain development: importance of genetic background.
The connexin43-dependent transcriptome during brain development: importance of genetic background.
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DOI：
10.1016/j.brainres.2012.05.062
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发表时间：
2012-12-03
期刊：
影响因子：
2.9
通讯作者：
Scemes E
中科院分区：
文献类型：
--
作者：
Iacobas S;Iacobas DA;Spray DC;Scemes E
使用零突变小鼠是评估特定蛋白质在大脑功能中作用的有效方法。在基因敲除小鼠身上进行的研究揭示了缝隙连接蛋白(连接蛋白)的一些意想不到的作用。因此，对连接蛋白43(Cx43)缺失脑中基因表达的分析表明，单个基因(Gja1)的缺失会导致位于所有染色体上的许多其他基因的表达水平发生变化，这些基因参与了广泛多样的功能途径。Cx43基因敲除和敲除的星形胶质细胞在基因表达水平、控制和协调方面的显著变化之间的显著重叠增加了Gja1代表基因调控网络的转录转录节点的可能性。然而，两个品系的小鼠星形胶质细胞中Gja1的条件性缺失导致了显著不同的表型。为了评估遗传背景对转录组的影响，我们对GFAP-Cre：Cx43f/f C57BL/6和129/SVEV小鼠的脑进行了基因芯片研究。在两个Cx43条件敲除中，Cx43核心基因的数量令人惊讶地低(在所有Cx43空体中都受到调控，而不受品系的影响)，而在两个Cx43条件敲除中有大量不同的调控基因，这表明小鼠品系对脑转录组的高度影响。
Use of null mutant mice is a powerful way to evaluate the role of specific proteins in brain function. Studies performed on knockout mice have revealed some unexpected roles of the gap junction proteins (connexins). Thus, analyses of gene expression in connexin43 (Cx43) null brains indicated that deletion of a single gene (Gja1) induced expression level change of numerous other genes located on all chromosomes and involved in a wide diversity of functional pathways. The significant overlap between alterations in gene expression level, control and coordination in Cx43 knockout and knockdown astrocytes raised the possibility that Gja1 represents a transcriptomic node of gene regulatory networks. However, conditional deletion of Gja1 in astrocytes of two mouse strains resulted in remarkably different phenotypes. In order to evaluate the influence of the genetic background on the transcriptome, we performed microarray studies on brains of GFAP-Cre:Cx43f/f C57Bl/6 and 129/SVEV mice. The surprisingly low number of Cx43 core genes (regulated in all Cx43 nulls regardless of strain) and the high number of differently regulated genes in the two Cx43 conditional knockouts indicate high influence of mouse strain on brain transcriptome.
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影响因子：
2.4
作者：
Iacobas, Dumitru A.;Suadicani, Sylvia O.;Scemes, Eliana
通讯作者：
Scemes, Eliana
影响因子：
2.4
作者：
Spray, David C.;Iacobas, Dumitru A.
通讯作者：
Iacobas, Dumitru A.
DOI：
10.1523/jneurosci.0341-09.2009
发表时间：
2009-06-17
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Lutz SE;Zhao Y;Gulinello M;Lee SC;Raine CS;Brosnan CF
通讯作者：
Brosnan CF
影响因子：
2.9
作者：
Iacobas, D. A.;Iacobas, S.;Spray, D. C.
通讯作者：
Spray, D. C.
影响因子：
6.2
作者：
Wiencken-Barger, Amy E.;Djukic, Biljana;McCarthy, Ken D.
通讯作者：
McCarthy, Ken D.