Intravenous grafts of amniotic fluid-derived stem cells induce endogenous cell proliferation and attenuate behavioral deficits in ischemic stroke rats.
Intravenous grafts of amniotic fluid-derived stem cells induce endogenous cell proliferation and attenuate behavioral deficits in ischemic stroke rats.
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DOI：
10.1371/journal.pone.0043779
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Borlongan CV
中科院分区：
文献类型：
--
作者：
Tajiri N;Acosta S;Glover LE;Bickford PC;Jacotte Simancas A;Yasuhara T;Date I;Solomita MA;Antonucci I;Stuppia L;Kaneko Y;Borlongan CV
我们最近报道了存活的大鼠羊水来源干细胞的分离。在这里，我们测试了AFS细胞在缺血性中风啮齿动物模型中的治疗效果。成年雄性SD大鼠大脑中动脉闭塞(MCAO)60min。35天后，表现出严重运动缺陷的动物接受了大鼠AFS细胞或载体的静脉移植。在大脑中动脉阻塞后60-63天，与注入车辆的中风动物相比，移植了AFS细胞的中风动物的运动和认知功能显著恢复。苏木精-伊红(H&E)染色显示，与注入载体的卒中动物相比，移植AFS细胞的卒中动物的齿状回(DG)和脑室下区(SVZ)的梗死体积显著缩小，细胞增殖标志Ki67和神经元标志MAP2显著增加。移植AFS细胞的卒中动物DG和SVZ的Ki67/MAP2双标阳性细胞数和Ki67/MAP2双标细胞数显著高于输注载体的卒中动物。本研究报告了AFS细胞移植对中风动物的治疗潜力，可能是通过增强内源性修复机制。
We recently reported isolation of viable rat amniotic fluid-derived stem (AFS) cells. Here, we tested the therapeutic benefits of AFS cells in a rodent model of ischemic stroke. Adult male Sprague-Dawley rats received a 60-minute middle cerebral artery occlusion (MCAo). Thirty-five days later, animals exhibiting significant motor deficits received intravenous transplants of rat AFS cells or vehicle. At days 60–63 post-MCAo, significant recovery of motor and cognitive function was seen in stroke animals transplanted with AFS cells compared to vehicle-infused stroke animals. Infarct volume, as revealed by hematoxylin and eosin (H&E) staining, was significantly reduced, coupled with significant increments in the cell proliferation marker, Ki67, and the neuronal marker, MAP2, in the dentate gyrus (DG) and the subventricular zone (SVZ) of AFS cell-transplanted stroke animals compared to vehicle-infused stroke animals. A significantly higher number of double-labeled Ki67/MAP2-positive cells and a similar trend towards increased Ki67/MAP2 double-labeling were observed in the DG and SVZ of AFS cell-transplanted stroke animals, respectively, compared to vehicle-infused stroke animals. This study reports the therapeutic potential of AFS cell transplantation in stroke animals, possibly via enhancement of endogenous repair mechanisms.
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影响因子：
3.3
作者：
Chopp M;Steinberg GK;Kondziolka D;Lu M;Bliss TM;Li Y;Hess DC;Borlongan CV
通讯作者：
Borlongan CV
影响因子：
3.3
作者：
Fukunaga, A;Uchida, K;Kawase, T
通讯作者：
Kawase, T
影响因子：
1.9
作者：
GALLAGHER, M;BURWELL, RD;BURCHINAL, M
通讯作者：
BURCHINAL, M
影响因子：
8.3
作者：
Borlongan, CV;Hadman, M;Sanberg, PR
通讯作者：
Sanberg, PR
影响因子：
1.9
作者：
Freret, Thomas;Bouet, Valentine;Boulouard, Michel
通讯作者：
Boulouard, Michel