Molecular evaluation of the metabolism of estrogenic di(2-ethylhexyl) phthalate in Mycolicibacterium sp.
Molecular evaluation of the metabolism of estrogenic di(2-ethylhexyl) phthalate in Mycolicibacterium sp.
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分枝杆菌中雌激素邻苯二甲酸二（2-乙基己基）酯代谢的分子评价。
DOI：
10.1186/s12934-023-02096-0
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发表时间：
2023-04-27
影响因子：
6.4
通讯作者：
Dutta, Tapan K. K.
中科院分区：
文献类型：
--
作者：
Bhattacharyya, Mousumi;Dhar, Rinita;Basu, Suman;Das, Avijit;Reynolds, Darren M. M.;Dutta, Tapan K. K.
邻苯二甲酸二（2-乙基己基）酯（DEHP）是一种被广泛检测到的增塑剂，也是一种因其对人类、野生动物和环境的不利影响而备受关注的优先污染物。为了消除这种有毒负担，生物过程是在生态友好条件下对抗猖獗的环境侮辱的最有前途的方法。本研究调查了Mycolicibacterium sp.菌株MBM同化雌激素DEHP的分解代谢潜力的生化和分子评估。一项详细的生物化学研究揭示了DEHP降解的初始水解途径，随后将水解的邻苯二甲酸和2-乙基己醇同化为TCA循环中间体。除了DEHP分解代谢酶的诱导性质外，菌株MBM还可以有效利用各种低分子量和高分子量的邻苯二甲酸二酯，并可以在中等耐盐条件下生长。全基因组序列分析显示基因组大小为6.2 Mb，GC含量为66.51%，包含6，878个编码序列，包括多个基因，注释为与邻苯二甲酸酯（PAEs）的催化剂相关。通过转录组评估和随后的RT-qPCR分析证实了注释基因，揭示了上调基因/基因簇在DEHP代谢中的可能作用，在分子水平上加强了降解的生化途径。生物化学、基因组学、转录组学和RT-qPCR分析的详细相关性突出了菌株MBM中的PAE降解分解代谢机制。此外，由于在淡水和海水的盐度范围内的功能属性，菌株MBM可以用作PAEs生物修复中的合适候选物。在线版本包含补充材料，可通过10.1186/s12934-023-02096-0获得。
Di(2-ethylhexyl) phthalate (DEHP) is a widely detected plasticizer and a priority pollutant of utmost concern for its adverse impact on humans, wildlife and the environment. To eliminate such toxic burden, biological processes are the most promising ways to combat rampant environmental insults under eco-friendly conditions. The present study investigated the biochemical and molecular assessment of the catabolic potential of Mycolicibacterium sp. strain MBM in the assimilation of estrogenic DEHP. A detailed biochemical study revealed an initial hydrolytic pathway of degradation for DEHP followed by the assimilation of hydrolyzed phthalic acid and 2-ethylhexanol to TCA cycle intermediates. Besides the inducible nature of DEHP-catabolic enzymes, strain MBM can efficiently utilize various low- and high-molecular-weight phthalate diesters and can grow under moderately halotolerant conditions. Whole genome sequence analysis exhibited a genome size of 6.2 Mb with a GC content of 66.51% containing 6,878 coding sequences, including multiple genes, annotated as relevant to the catabolism of phthalic acid esters (PAEs). Substantiating the annotated genes through transcriptome assessment followed by RT-qPCR analysis, the possible roles of upregulated genes/gene clusters in the metabolism of DEHP were revealed, reinforcing the biochemical pathway of degradation at the molecular level. A detailed co-relation of biochemical, genomic, transcriptomic and RT-qPCR analyses highlights the PAE-degrading catabolic machineries in strain MBM. Further, due to functional attributes in the salinity range of both freshwater and seawater, strain MBM may find use as a suitable candidate in the bioremediation of PAEs. The online version contains supplementary material available at 10.1186/s12934-023-02096-0.
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影响因子：
11.4
作者：
Chen, Chih-Yu;Wu, Pei-Ssu;Chung, Ying-Chien
通讯作者：
Chung, Ying-Chien
影响因子：
6.7
作者：
Golubev, Sergey N.;Muratova, Anna Yu.;Turkovskaya, Olga, V
通讯作者：
Turkovskaya, Olga, V
影响因子：
5.2
作者：
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通讯作者：
Son J
影响因子：
4.5
作者：
Hu T;Yang C;Hou Z;Liu T;Mei X;Zheng L;Zhong W
通讯作者：
Zhong W
影响因子：
4.6
作者：
Everaert C;Luypaert M;Maag JLV;Cheng QX;Dinger ME;Hellemans J;Mestdagh P
通讯作者：
Mestdagh P