Tear film lipids.
Tear film lipids.
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DOI：
10.1016/j.exer.2013.05.010
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发表时间：
2013-12
影响因子：
3.4
通讯作者：
Butovich, Igor A.
中科院分区：
文献类型：
--
作者：
Butovich, Igor A.
人类眉毛腺分泌物是由不同类型的脂类组成的复杂混合物，如蜡酯、胆固醇酯、(O-酰基)-ω-羟基脂肪酸及其酯、酰甘油、二酰化二醇、游离脂肪酸、胆固醇和少量其他极性和非极性脂类，它们的化学性质和在MGS中的存在一直存在激烈的争论。本综述的目的是讨论使用不同的实验技术获得的最新结果，估计其可用性的局限性，并讨论它们的生化、生物物理和生理意义。为了创建MGS和眼泪的脂谱，作者在实验室获得的结果与关于MGS和眼泪化学成分的现有信息相结合。详细讨论了当今研究人员可用的最具信息量的方法，如高效液相色谱-质谱仪、气相色谱-质谱仪和质子核磁共振。给出了一张眉目脂质体图(如在反相液相色谱/质谱学实验中所见)。概述了该领域未来努力的方向。
Human meibomian gland secretions (MGS, or meibum) are formed from a complex mixture of lipids of different classes such as wax esters, cholesteryl esters, (O-acyl)-ω-hydroxy fatty acids (OAHFA) and their esters, acylglycerols, diacylated diols, free fatty acids, cholesterol, and a smaller amount of other polar and nonpolar lipids, whose chemical nature and the very presence in MGS have been a matter of intense debates. The purpose of this review is to discuss recent results that were obtained using different experimental techniques, estimate limitations of their usability, and discuss their biochemical, biophysical, and physiological implications. To create a lipid map of MGS and tears, the results obtained in the author’s laboratory were integrated with available information on chemical composition of MGS and tears. The most informative approaches that are available today to researchers, such as HPLC-MS, GC-MS, and proton NMR, are discussed in details. A map of the meibomian lipidome (as it is seen in reverse phase liquid chromatography/mass spectrometry experiments) is presented. Directions of future efforts in the area are outlined.
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影响因子：
4.4
作者：
Arciniega, Juan C.;Uchiyama, Eduardo;Butovich, Igor A.
通讯作者：
Butovich, Igor A.
影响因子：
4.2
作者：
Carrott, MJ;Jones, DC;Davidson, G
通讯作者：
Davidson, G
影响因子：
4.4
作者：
Borchman, Douglas;Foulks, Gary N.;Milliner, Sarah E.
通讯作者：
Milliner, Sarah E.
影响因子：
4.4
作者：
Butovich, Igor A.;Arciniega, Juan C.;Molai, Mike
通讯作者：
Molai, Mike
DOI：
10.1007/978-1-60761-322-0_11
发表时间：
2009-01-01
期刊：
LIPIDOMICS: VOL 1: METHODS AND PROTOCOLS
影响因子：
--
作者：
Butovich, Igor A.
通讯作者：
Butovich, Igor A.