Immune responses to plague infection in wild Rattus rattus, in Madagascar: a role in foci persistence?
Immune responses to plague infection in wild Rattus rattus, in Madagascar: a role in foci persistence?
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DOI：
10.1371/journal.pone.0038630
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Jambou R
中科院分区：
文献类型：
--
作者：
Andrianaivoarimanana V;Telfer S;Rajerison M;Ranjalahy MA;Andriamiarimanana F;Rahaingosoamamitiana C;Rahalison L;Jambou R
鼠疫是马达加斯加中部高地的地方病，其主要宿主是黑鼠。通常认为该物种易感染鼠疫，感染后迅速死亡，导致受感染跳蚤的传播，因此将疾病传播给人类。然而，在同一地区的传播焦点，从一年到一年的持续性，假设的维持机制，其中大鼠的免疫反应可能发挥主要作用。鼠疫的免疫力和鼠的存活率对鼠疫疫源地的稳定起重要作用。在这项研究中，我们的目的是调查血清学反应鼠疫在马达加斯加流行区的野生黑鼠。此外，我们评估了使用最近开发的快速血清学诊断试验，以调查潜在的水库宿主在鼠疫疫源地的免疫反应。我们用鼠疫耶尔森氏菌实验性感染野鼠，以研究短期和长期的抗体应答。检测抗F1 IgM和IgG以评价这种抗体应答。高水平的抗F1 IgM和IgG被发现在大鼠分别在一个和三个星期后的挑战，反应有很大差异的村庄。分别接种500和1500菌落形成单位（cfu）时，抗F1 IgM和IgG应答达到平台。超过10%的大鼠能够维持抗F1反应超过一年。使用试纸方便地跟踪这种抗F1应答。接种极少量的细菌就足以在野鼠中诱导高免疫应答，使它们在感染后得以存活。村内和村间鼠免疫反应存在很大的异质性，这可能对鼠疫流行病学产生严重影响。此外，结果表明，在现场，抗F1试纸是有效的调查鼠疫疫情传播后几个月。
Plague is endemic within the central highlands of Madagascar, where its main reservoir is the black rat, Rattus rattus. Typically this species is considered susceptible to plague, rapidly dying after infection inducing the spread of infected fleas and, therefore, dissemination of the disease to humans. However, persistence of transmission foci in the same area from year to year, supposes mechanisms of maintenance among which rat immune responses could play a major role. Immunity against plague and subsequent rat survival could play an important role in the stabilization of the foci. In this study, we aimed to investigate serological responses to plague in wild black rats from endemic areas of Madagascar. In addition, we evaluate the use of a recently developed rapid serological diagnostic test to investigate the immune response of potential reservoir hosts in plague foci. We experimentally infected wild rats with Yersinia pestis to investigate short and long-term antibody responses. Anti-F1 IgM and IgG were detected to evaluate this antibody response. High levels of anti-F1 IgM and IgG were found in rats one and three weeks respectively after challenge, with responses greatly differing between villages. Plateau in anti-F1 IgM and IgG responses were reached for as few as 500 and 1500 colony forming units (cfu) inoculated respectively. More than 10% of rats were able to maintain anti-F1 responses for more than one year. This anti-F1 response was conveniently followed using dipsticks. Inoculation of very few bacteria is sufficient to induce high immune response in wild rats, allowing their survival after infection. A great heterogeneity of rat immune responses was found within and between villages which could heavily impact on plague epidemiology. In addition, results indicate that, in the field, anti-F1 dipsticks are efficient to investigate plague outbreaks several months after transmission.
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影响因子：
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