Actinomycete integrative and conjugative elements.
Actinomycete integrative and conjugative elements.
复制标题
放线菌整合和共轭元素。
DOI：
10.1007/s10482-008-9255-x
复制
发表时间：
2008-06
影响因子：
2.6
通讯作者：
Dijkhuizen, Lubbert
中科院分区：
文献类型：
--
作者：
Poele, Evelien M. te;Bolhuis, Henk;Dijkhuizen, Lubbert
关键词：
综述了放线菌整合接合元件的研究进展。最佳表征的AICE，两性链霉菌的pSAM 2（10.9 kb）、天蓝色链霉菌的SLP 1（17.3 kb）和拟甲醇无枝酸菌的pMEA 300（13.3 kb），作为整合元件存在于特定的tRNA基因中，并且能够接合转移。这些AICE具有高度保守的结构组织，具有用于切除/整合、复制、接合转移和调节的功能模块。最近，已经表明，基于它们的复制起始蛋白RepAM的独特特征，地中海拟无枝酸菌的pMEA 300和相关元件pMEA 100以及葡萄多孢菌的pSE 211形成了一组新的AICE，即pMEA元件。对大量拟无枝酸菌属分离株的评价允许鉴定多种pMEA样元件。我们的数据表明，作为AICE，它们主要以整合的形式与自然宿主共同进化，而不是通过水平基因转移分散。pMEA样元件可以分为两个不同的群体，来自不同的地理来源。一组与pMEA 300最密切相关，发现于来自澳大利亚和亚洲的分离株中，pMEA 100相关序列存在于欧洲分离株中。基因组序列数据极大地促进了最近的洞察力，AICE存在于许多放线菌属。序列数据还提供了对它们进化关系的更多了解，揭示了它们的模块组成以及它们可能来自细菌质粒和噬菌体的组合血统。越来越多的证据表明，AICE作为宿主基因组多样性的调节剂，也参与通过水平基因转移获得次级代谢物簇和外源DNA。尽管仍处于推测阶段，但这些AICE可能在抗生素耐药因子向致病菌的传播中发挥作用。本综述中提出的关于AICE特征的新见解可用于有效构建新载体，使我们能够设计和优化菌株，用于生产商业和医学上感兴趣的次级代谢产物和生物活性蛋白。
This paper reviews current knowledge on actinomycete integrative and conjugative elements (AICEs). The best characterised AICEs, pSAM2 of Streptomyces ambofaciens (10.9 kb), SLP1 (17.3 kb) of Streptomyces coelicolor and pMEA300 of Amycolatopsis methanolica (13.3 kb), are present as integrative elements in specific tRNA genes, and are capable of conjugative transfer. These AICEs have a highly conserved structural organisation, with functional modules for excision/integration, replication, conjugative transfer, and regulation. Recently, it has been shown that pMEA300 and the related elements pMEA100 of Amycolatopsis mediterranei and pSE211 of Saccharopolyspora erythraea form a novel group of AICEs, the pMEA-elements, based on the unique characteristics of their replication initiator protein RepAM. Evaluation of a large collection of Amycolatopsis isolates has allowed identification of multiple pMEA-like elements. Our data show that, as AICEs, they mainly coevolved with their natural host in an integrated form, rather than being dispersed via horizontal gene transfer. The pMEA-like elements could be separated into two distinct populations from different geographical origins. One group was most closely related to pMEA300 and was found in isolates from Australia and Asia and pMEA100-related sequences were present in European isolates. Genome sequence data have enormously contributed to the recent insight that AICEs are present in many actinomycete genera. The sequence data also provide more insight into their evolutionary relationships, revealing their modular composition and their likely combined descent from bacterial plasmids and bacteriophages. Evidence is accumulating that AICEs act as modulators of host genome diversity and are also involved in the acquisition of secondary metabolite clusters and foreign DNA via horizontal gene transfer. Although still speculative, these AICEs may play a role in the spread of antibiotic resistance factors into pathogenic bacteria. The novel insights on AICE characteristics presented in this review may be used for the effective construction of new vectors that allows us to engineer and optimise strains for the production of commercially and medically interesting secondary metabolites, and bioactive proteins.
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影响因子：
3.2
作者：
BRASCH, MA;COHEN, SN
通讯作者：
COHEN, SN
影响因子：
3.2
作者：
BROWN, DP;CHIANG, SJD;KATZ, L
通讯作者：
KATZ, L
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3.5
作者：
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通讯作者：
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2.6
作者：
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通讯作者：
WRIGHT, HM
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3.2
作者：
BEGG, KJ;DEWAR, SJ;DONACHIE, WD
通讯作者：
DONACHIE, WD