Next generation sequencing and de novo transcriptome analysis of Costus pictus D. Don, a non-model plant with potent anti-diabetic properties.
Next generation sequencing and de novo transcriptome analysis of Costus pictus D. Don, a non-model plant with potent anti-diabetic properties.
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DOI：
10.1186/1471-2164-13-663
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发表时间：
2012-11-23
期刊：
影响因子：
4.4
通讯作者：
Rao SN
中科院分区：
文献类型：
--
作者：
Annadurai RS;Jayakumar V;Mugasimangalam RC;Katta MA;Anand S;Gopinathan S;Sarma SP;Fernandes SJ;Mullapudi N;Murugesan S;Rao SN
除其他糖尿病控制措施外，植物疗法在II型糖尿病（DM）患者中很受欢迎。已知许多植物物种具有控制糖尿病的特性。木香树在印度南部被称为“胰岛素植物”，据报道，它的叶子可以增加血浆中的胰岛素池。下一代测序是鉴定与植物组织生理功能相关的转录组分子特征的有力工具。我们使用Illumina可逆染料终止测序技术对C. pictus叶片转录组进行测序，并结合生物信息学工具鉴定C. pictus抗糖尿病特性相关转录本。共鉴定出55,006个转录本，其中69.15%的转录本可被注释。我们从类异戊二烯次生代谢物中鉴定出了与碧馨生物合成途径、香叶醇和香叶生物合成途径相关的转录本，并验证了碧馨前体——降碧馨甲基转移酶的存在。已知编码这些萜类化合物的转录本是过氧化物酶体增殖激活受体（PPAR）激动剂和抗糖基化剂。序贯提取和高效液相色谱法证实了碧心草甲醇提取物的存在。另一个与抗糖尿病、抗肥胖和免疫调节相关的重要转录物是脱落酸生物合成途径。我们还报道了许多其他的转录物的生物合成抗肿瘤，抗氧化和抗菌的代谢物。报道了桂树叶片抗糖尿病特性的固体分子特征（与碧馨、脱落酸、香叶和香叶醇的生物合成相关的转录本），以及通过次级代谢物途径注释与其他植物化学功能如抗肿瘤、抗氧化、免疫调节、抗微生物和抗疟疾特性相关的重要线索。所提供的数据将对使用草药治疗糖尿病的研究人员有巨大的帮助。
Phyto-remedies for diabetic control are popular among patients with Type II Diabetes mellitus (DM), in addition to other diabetic control measures. A number of plant species are known to possess diabetic control properties. Costus pictus D. Don is popularly known as “Insulin Plant” in Southern India whose leaves have been reported to increase insulin pools in blood plasma. Next Generation Sequencing is employed as a powerful tool for identifying molecular signatures in the transcriptome related to physiological functions of plant tissues. We sequenced the leaf transcriptome of C. pictus using Illumina reversible dye terminator sequencing technology and used combination of bioinformatics tools for identifying transcripts related to anti-diabetic properties of C. pictus. A total of 55,006 transcripts were identified, of which 69.15% transcripts could be annotated. We identified transcripts related to pathways of bixin biosynthesis and geraniol and geranial biosynthesis as major transcripts from the class of isoprenoid secondary metabolites and validated the presence of putative norbixin methyltransferase, a precursor of Bixin. The transcripts encoding these terpenoids are known to be Peroxisome Proliferator-Activated Receptor (PPAR) agonists and anti-glycation agents. Sequential extraction and High Performance Liquid Chromatography (HPLC) confirmed the presence of bixin in C. pictus methanolic extracts. Another significant transcript identified in relation to anti-diabetic, anti-obesity and immuno-modulation is of Abscisic Acid biosynthetic pathway. We also report many other transcripts for the biosynthesis of antitumor, anti-oxidant and antimicrobial metabolites of C. pictus leaves. Solid molecular signatures (transcripts related to bixin, abscisic acid, and geranial and geraniol biosynthesis) for the anti-diabetic properties of C. pictus leaves and vital clues related to the other phytochemical functions like antitumor, anti-oxidant, immuno-modulatory, anti-microbial and anti-malarial properties through the secondary metabolite pathway annotations are reported. The data provided will be of immense help to researchers working in the treatment of DM using herbal therapies.
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影响因子：
4.4
作者：
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通讯作者：
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作者：
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