Heat-solubilized curry spice curcumin inhibits antibody-antigen interaction in in vitro studies: a possible therapy to alleviate autoimmune disorders.
Heat-solubilized curry spice curcumin inhibits antibody-antigen interaction in in vitro studies: a possible therapy to alleviate autoimmune disorders.
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在体外研究中，热溶性咖喱香料姜黄素抑制抗体-抗原相互作用：一种缓解自身免疫性疾病的可能疗法。
DOI：
10.1002/mnfr.200900106
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发表时间：
2010
影响因子：
5.2
通讯作者：
Scofield,RHal
中科院分区：
文献类型：
--
作者：
Kurien,BijiT;D'Souza,Anil;Scofield,RHal
慢性和复杂的自身免疫性疾病，目前用免疫抑制剂姑息治疗，需要多靶向治疗以获得更大的疗效。天然存在的多酚姜黄素已成为一种强大的“营养药物”，与多个目标相互作用，以安全和廉价地消退疾病。高达8克/天的姜黄素18个月对人类无毒。然而，姜黄素的效用受到其水不溶性的限制。我们已经证明了热介导的姜黄素的水溶性增加了12倍。在这里，我们通过SDS-PAGE和表面等离子体共振显示热增溶的姜黄素与蛋白质结合。基于这种结合，我们假设热溶解的姜黄素或姜黄会阻止同源自身抗原的自身抗体靶向。热溶解姜黄素/姜黄显着降低自身抗体的结合从干燥综合征（高达43/70%，分别）和系统性红斑狼疮（高达52/70%，分别）患者以及干燥综合征的动物模型（高达50/60%，分别），其同源抗原。然而，抑制不是特异性的自身免疫。热溶解姜黄素/姜黄还抑制商业多克隆抗血影蛋白与血影蛋白的结合（分别为50/56%）。因此，我们认为多方面的热溶解姜黄素可以改善自身免疫性疾病。此外，无毒的姜黄素可以作为一种新的蛋白质染色在SDS-PAGE，即使它是不敏感的考马斯系统，涉及有毒化学品。
Chronic and complex autoimmune diseases, currently treated palliatively with immunosuppressives, require multi-targeted therapy for greater effectiveness. The naturally occurring polyphenol curcumin has emerged as a powerful ‘‘nutraceutical’’that interacts with multiple targets to regress diseases safely and inexpensively. Up to 8 g/day of curcumin for 18 months was non-toxic to humans. However, curcumin’s utility is limited by its aqueous insolubility. We have demonstrated a heat-mediated 12-fold increase in curcumin’s aqueous solubility. Here, we show by SDS-PAGE and surface plasmon resonance that heat-solubilized curcumin binds to proteins. Based on this binding we hypothesized that heat-solubilized curcumin or turmeric would prevent autoantibody targeting of cognate autoantigens. Heat-solubilized curcumin/turmeric significantly decreased binding of autoantibodies from Sjögren’s syndrome (up to 43/70%, respectively) and systemic lupus erythematosus (up to 52/70%, respectively) patients as well as an animal model of Sjögren’s syndrome (up to 50/60%, respectively) to their cognate antigens. However, inhibition was not specific to autoimmunity. Heat-solubilized curcumin/turmeric also inhibited binding of commercial polyclonal antispectrin to spectrin (50/56%, respectively). Thus, we suggest that the multifaceted heatsolubilized curcumin can ameliorate autoimmune disorders. In addition, the non-toxic curcumin could serve as a new protein stain in SDS-PAGE even though it is less sensitive than the Coomassie system which involves toxic chemicals.
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有或没有蛋白质合成的培养胚胎鸡晶状体上皮细胞的伸长
影响因子：
7.8
作者：
J. Piatigorsky;H. Webster;M. Wollberg
通讯作者：
M. Wollberg
胚胎和成年鸡晶状体纤维细胞的表面形态。
DOI：
10.1002/aja.1001590406
发表时间：
1980
期刊：
The American journal of anatomy
影响因子：
--
作者：
J. Kuszak;J. Alcala;H. Maisel
通讯作者：
H. Maisel
DOI：
10.1016/0304-4157(76)90004-6
发表时间：
1976-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
BENEDETTI, EL;DUNIA, I;BLOEMENDAL, H
通讯作者：
BLOEMENDAL, H
影响因子：
33.6
作者：
U. Rutishauser;T. Jessell
通讯作者：
U. Rutishauser;T. Jessell
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影响因子：
3.4
作者：
J. Alcala;H. Maisel
通讯作者：
H. Maisel