Paired associated magnetic stimulation promotes neural repair in the rat middle cerebral artery occlusion model of stroke.
Paired associated magnetic stimulation promotes neural repair in the rat middle cerebral artery occlusion model of stroke.
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DOI：
10.4103/1673-5374.282266
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发表时间：
2020-11
影响因子：
6.1
通讯作者：
Bai YL
中科院分区：
文献类型：
--
作者：
Gao BY;Sun CC;Xia GH;Zhou ST;Zhang Y;Mao YR;Liu PL;Zheng Y;Zhao D;Li XT;Xu J;Xu DS;Bai YL
成对联想刺激已被用于中风患者作为一种创新的恢复治疗。然而，配对联想刺激对神经功能的治疗效果的机制仍不清楚。本研究将大鼠随机分为大脑中动脉阻塞模型组（MCAO）和配对联合磁刺激组（PAMS）。采用大脑中动脉栓塞法制备MCAO大鼠模型。PAMS组于MCAO后第3 ~ 20天接受PAMS治疗。MCAO组接受假刺激，每周3次。在缺血后18天内，对大鼠进行行为学实验-足错试验、平衡木行走试验和爬梯行走试验。PAMS组患者术后15、17 d平衡能力明显改善，术后15 d患肢足部失误率低于MCAO组。Western blot检测结果显示，与MCAO组相比，PAMS组大鼠感觉运动皮层脑源性神经营养因子、谷氨酸受体2/3、突触后密度蛋白95和突触素1的表达水平在第21天显著升高。静息状态功能磁共振成像显示，在同侧半球的感觉运动皮层的区域大脑活动增加，但在对侧半球的第20天下降。通过有限元模拟，与模板中的对侧皮层相比，缺血皮层中的电场分布显示出更高的强度，约为0.4 A/m2。总之，我们的研究结果表明，PAMS上调神经可塑性相关蛋白，增加区域脑活动，并促进受影响的感觉运动皮层在大鼠MCAO模型的功能恢复。实验于2018年3月3日获得中国复旦大学实验动物管理和使用委员会批准（批准号：201802173 S）。
Paired associative stimulation has been used in stroke patients as an innovative recovery treatment. However, the mechanisms underlying the therapeutic effectiveness of paired associative stimulation on neurological function remain unclear. In this study, rats were randomly divided into middle cerebral occlusion model (MCAO) and paired associated magnetic stimulation (PAMS) groups. The MCAO rat model was produced by middle cerebral artery embolization. The PAMS group received PAMS on days 3 to 20 post MCAO. The MCAO group received sham stimulation, three times every week. Within 18 days after ischemia, rats were subjected to behavioral experiments—the foot-fault test, the balance beam walking test, and the ladder walking test. Balance ability was improved on days 15 and 17, and the foot-fault rate was less in their affected limb on day 15 in the PAMS group compared with the MCAO group. Western blot assay showed that the expression levels of brain derived neurotrophic factor, glutamate receptor 2/3, postsynaptic density protein 95 and synapsin-1 were significantly increased in the PAMS group compared with the MCAO group in the ipsilateral sensorimotor cortex on day 21. Resting-state functional magnetic resonance imaging revealed that regional brain activities in the sensorimotor cortex were increased in the ipsilateral hemisphere, but decreased in the contralateral hemisphere on day 20. By finite element simulation, the electric field distribution showed a higher intensity, of approximately 0.4 A/m2, in the ischemic cortex compared with the contralateral cortex in the template. Together, our findings show that PAMS upregulates neuroplasticity-related proteins, increases regional brain activity, and promotes functional recovery in the affected sensorimotor cortex in the rat MCAO model. The experiments were approved by the Institutional Animal Care and Use Committee of Fudan University, China (approval No. 201802173S) on March 3, 2018.
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影响因子：
3.5
作者：
Lin, Yen-Nung;Hu, Chaur-Jong;Liou, Tsan-Hon
通讯作者：
Liou, Tsan-Hon
影响因子：
5.5
作者：
Cheeran, Binith;Talelli, Penelope;Rothwell, John C.
通讯作者：
Rothwell, John C.
影响因子：
6.6
作者：
Kozel, F. Andrew;Motes, Michael A.;Hart, John, Jr.
通讯作者：
Hart, John, Jr.
DOI：
10.1007/s00259-009-1342-3
发表时间：
2010-05-01
影响因子：
9.1
作者：
Gao, Feng;Wang, Shuang;Zhang, Hong
通讯作者：
Zhang, Hong
影响因子：
16.2
作者：
Diering GH;Huganir RL
通讯作者：
Huganir RL