Effect of docosahexaenoic acid supplementation on inflammatory cytokine levels in infants at high genetic risk for type 1 diabetes.
Effect of docosahexaenoic acid supplementation on inflammatory cytokine levels in infants at high genetic risk for type 1 diabetes.
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DOI：
10.1111/pedi.12170
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发表时间：
2015-06
影响因子：
3.4
通讯作者：
Type 1 Diabetes TrialNet Nutritional Intervention to Prevent (NIP) Type 1 Diabetes Study Group
中科院分区：
文献类型：
--
作者：
Chase HP;Boulware D;Rodriguez H;Donaldson D;Chritton S;Rafkin-Mervis L;Krischer J;Skyler JS;Clare-Salzler M;Type 1 Diabetes TrialNet Nutritional Intervention to Prevent (NIP) Type 1 Diabetes Study Group
1型糖尿病（T1 D）是由胰腺β细胞的炎症性破坏引起的。在本研究中，我们研究了补充二十二碳六烯酸（DHA）对具有高遗传风险的T1 D婴儿的白色血细胞（WBC）中刺激炎症细胞因子产生的影响。这是一项多中心、双臂、随机、双盲的DHA补充试验，从妊娠的最后三个月（41名婴儿）或出生后的前五个月（57名婴儿）开始。通过气相色谱/质谱法分析了婴儿和母亲红细胞（RBC）膜和母乳中的DHA水平。在用高剂量脂多糖（LPS）（1μg/mL）刺激后，使用Luminex Multiplex测定法从全血培养上清液中测定炎性细胞因子。在6至36个月的婴儿中，与对照组相比，治疗组的RBC DHA水平增加了61-100%。在测量的6个时间点的任何时间点，炎性细胞因子IL-1β、TNFα或IL-12 p40的产生均未出现统计学显著性降低。在12个月大时，母乳喂养的DHA治疗婴儿的炎症标志物hsCRP显著低于所有配方奶粉喂养的婴儿。3名婴儿（2名接受DHA）因出现≥ 2种持续阳性生化胰岛自身抗体而从研究中排除。这项初步试验表明，在婴儿饮食中补充DHA是安全的，并实现了将婴儿RBC DHA水平提高至少20%的研究前目标。炎性细胞因子的产生并没有持续减少。
Type 1 diabetes (T1D) results from the inflammatory destruction of pancreatic β-cells. In the present study, we investigated the effect of docosahexaenoic acid (DHA) supplementation on stimulated inflammatory cytokine production in white blood cells (WBC) from infants with a high genetic risk for T1D. This was a multicenter, two-arm, randomized, double blind pilot trial of DHA supplementation, beginning either in the last trimester of pregnancy (41 infants) or in the first five months after birth (57 infants). Levels of DHA in infant and maternal red blood cell (RBC) membranes and in breast milk were analyzed by gas chromatography/mass spectrometry. Inflammatory cytokines were assayed from whole blood culture supernatants using the Luminex Multiplex assay after stimulation with high dose lipopolysaccharide (LPS), 1μg/mL. The levels of RBC DHA were increased by 61–100% in treated compared to control infants at ages 6 to 36 months. There were no statistically significant reductions in production of the inflammatory cytokines, IL-1β, TNFα or IL-12p40 at any of the 6 time points measured. The inflammatory marker, hsCRP, was significantly lower in breast-fed DHA-treated infants compared to all formula-fed infants at age 12 months. Three infants (two received DHA) were removed from the study as a result of developing ≥ two persistently positive biochemical islet autoantibodies. This pilot trial showed that supplementation of infant diets with DHA is safe and fulfilled the pre-study goal of increasing infant RBC DHA levels by at least 20%. Inflammatory cytokine production was not consistently reduced.
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影响因子：
16.2
作者：
Vehik K;Hamman RF;Lezotte D;Norris JM;Klingensmith GJ;Rewers M;Dabelea D
通讯作者：
Dabelea D
影响因子：
16.2
作者：
Mayer-Davis EJ;Dabelea D;Crandell JL;Crume T;D'Agostino RB Jr;Dolan L;King IB;Lawrence JM;Norris JM;Pihoker C;The N
通讯作者：
The N
影响因子：
8.2
作者：
Åkerblom, HK;Virtanen, SM;Paronen, J
通讯作者：
Paronen, J
影响因子：
4.5
作者：
Berger, Alvin;Mutch, David M;Roberts, Matthew A
通讯作者：
Roberts, Matthew A
影响因子：
3.6
作者：
VANHOUWELINGEN, AC;SORENSEN, JD;SECHER, NJ
通讯作者：
SECHER, NJ