The Medaka Inbred Kiyosu-Karlsruhe (MIKK) panel.
The Medaka Inbred Kiyosu-Karlsruhe (MIKK) panel.
复制标题
DOI：
10.1186/s13059-022-02623-z
复制
发表时间：
2022-02-21
期刊：
影响因子：
12.3
通讯作者：
Loosli F
中科院分区：
文献类型：
--
作者：
Fitzgerald T;Brettell I;Leger A;Wolf N;Kusminski N;Monahan J;Barton C;Herder C;Aadepu N;Gierten J;Becker C;Hammouda OT;Hasel E;Lischik C;Lust K;Sokolova N;Suzuki R;Tsingos E;Tavhelidse T;Thumberger T;Watson P;Welz B;Khouja N;Naruse K;Birney E;Wittbrodt J;Loosli F
解开遗传变异和表型性状之间的关系仍然是生物学的一个基本挑战。在控制混杂环境因素的同时，映射复杂性状的变异往往是有问题的。为了解决这个问题，我们建立了一个脊椎动物的遗传资源，专门允许强大的基因型表型调查。青鳉（Oryzias latipes）是一种具有悠久遗传学研究历史的遗传模式系统，具有很强的抗近交能力。在这里，我们提出了青鳉近交Kiyosu-Karlsruhe（MIKK）面板：第一个近等基因面板的80个近交系的脊椎动物模型来自野生创始人人口。近交系提供了固定的基因组，这是重复研究、以受控方式改变遗传和环境的研究以及功能测试的先决条件。因此，MIKK小组将使复杂遗传性状的表型与基因型关联研究成为可能，同时允许仔细控制相互作用的因素，在遗传研究，人类健康，药物开发和基础生物学中有许多应用。在这里，我们提出了一个详细的描述整个MIKK面板的遗传变异，它提供了一个丰富的和独特的遗传资源的社区，使大规模的实验映射复杂的性状。在线版本包含补充材料，可通过10.1186/s13059-022-02623-z获得。
Unraveling the relationship between genetic variation and phenotypic traits remains a fundamental challenge in biology. Mapping variants underlying complex traits while controlling for confounding environmental factors is often problematic. To address this, we establish a vertebrate genetic resource specifically to allow for robust genotype-to-phenotype investigations. The teleost medaka (Oryzias latipes) is an established genetic model system with a long history of genetic research and a high tolerance to inbreeding from the wild. Here we present the Medaka Inbred Kiyosu-Karlsruhe (MIKK) panel: the first near-isogenic panel of 80 inbred lines in a vertebrate model derived from a wild founder population. Inbred lines provide fixed genomes that are a prerequisite for the replication of studies, studies which vary both the genetics and environment in a controlled manner, and functional testing. The MIKK panel will therefore enable phenotype-to-genotype association studies of complex genetic traits while allowing for careful control of interacting factors, with numerous applications in genetic research, human health, drug development, and fundamental biology. Here we present a detailed characterization of the genetic variation across the MIKK panel, which provides a rich and unique genetic resource to the community by enabling large-scale experiments for mapping complex traits. The online version contains supplementary material available at 10.1186/s13059-022-02623-z.
登录
查看更多内容
影响因子：
56.9
作者：
Ganna, Andrea;Verweij, Karin J. H.;Wilson, C. H.
通讯作者：
Wilson, C. H.
影响因子：
9.2
作者：
Chang CC;Chow CC;Tellier LC;Vattikuti S;Purcell SM;Lee JJ
通讯作者：
Lee JJ
影响因子：
64.5
作者：
Astle, William J.;Elding, Heather;Soranzo, Nicole
通讯作者：
Soranzo, Nicole
影响因子：
9.3
作者：
Ashbrook DG;Arends D;Prins P;Mulligan MK;Roy S;Williams EG;Lutz CM;Valenzuela A;Bohl CJ;Ingels JF;McCarty MS;Centeno AG;Hager R;Auwerx J;Lu L;Williams RW
通讯作者：
Williams RW
影响因子：
30.8
作者：
Braasch I;Gehrke AR;Smith JJ;Kawasaki K;Manousaki T;Pasquier J;Amores A;Desvignes T;Batzel P;Catchen J;Berlin AM;Campbell MS;Barrell D;Martin KJ;Mulley JF;Ravi V;Lee AP;Nakamura T;Chalopin D;Fan S;Wcisel D;Cañestro C;Sydes J;Beaudry FE;Sun Y;Hertel J;Beam MJ;Fasold M;Ishiyama M;Johnson J;Kehr S;Lara M;Letaw JH;Litman GW;Litman RT;Mikami M;Ota T;Saha NR;Williams L;Stadler PF;Wang H;Taylor JS;Fontenot Q;Ferrara A;Searle SM;Aken B;Yandell M;Schneider I;Yoder JA;Volff JN;Meyer A;Amemiya CT;Venkatesh B;Holland PW;Guiguen Y;Bobe J;Shubin NH;Di Palma F;Alföldi J;Lindblad-Toh K;Postlethwait JH
通讯作者：
Postlethwait JH