Real-Space Investigation of Energy Transfer through Electron Tomography
Real-Space Investigation of Energy Transfer through Electron Tomography
复制标题
通过电子断层扫描进行能量转移的真实空间研究
DOI：
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
N. Hildebrandt
中科院分区：
文献类型：
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作者：
Y. Ishida;Ikumi Akita;T. Pons;T. Yonezawa;N. Hildebrandt
本文报道了通过高分辨率电子断层扫描观察两种不同类型量子点（QD）超分子组件的三维构型，首次对它们的福斯特共振能量转移（FRET）进行了实空间研究。由于其在光合作用、人工光收集天线和蛋白质-蛋白质相互作用研究中的关键作用，FRET的机制已经通过各种光谱技术监测其激发态动力学进行了深入研究。利用电子断层扫描技术可以直接定位自组装纳米结构中单个量子点的三维坐标，并从理论上估计单个荧光团、结构域或超分子组装的FRET效率。此外，荧光光谱测定的FRET效率的实验值与通过电子断层扫描得到的幅度很好地一致。我们认为，所描述的策略可以用于单m…
We herein report the first real-space investigation of Forster resonance energy transfer (FRET) in two different types of quantum dot (QD) supramolecular assemblies by observing their three-dimensional (3D) configurations through high-resolution electron tomography. Owing to its critical role in photosynthesis, artificial light-harvesting antennas, and investigation of protein–protein interactions, the mechanism of FRET has been intensively studied by monitoring its excited-state dynamics via various spectroscopic techniques. The utilized electron tomography technique allowed the direct localization of 3D coordinates of individual QDs in self-assembled nanostructures and theoretical estimation of the FRET efficiency of a single fluorophore, domain, or supramolecular assembly. Moreover, the experimental value of the FRET efficiency determined by fluorescence spectroscopy was in good agreement with the magnitude obtained via electron tomography. We believe that the described strategy can be used in single-m...
DOI：
10.1002/anie.201611288
发表时间：
2017-03-06
期刊：
Angewandte Chemie (International ed. in English)
影响因子：
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作者：
Sanchez-Gaytan BL;Fay F;Hak S;Alaarg A;Fayad ZA;Pérez-Medina C;Mulder WJ;Zhao Y
通讯作者：
Zhao Y
影响因子：
41.2
作者：
Jia, C. L.;Mi, S. B.;Thust, A.
通讯作者：
Thust, A.