Metabolic Roles of a Rhodobacter sphaeroides Member of the ς32 Family
Metabolic Roles of a Rhodobacter sphaeroides Member of the ς32 Family
复制标题
δ32 家族成员球形红杆菌的代谢作用
DOI：
--
复制
发表时间：
1998
期刊：
影响因子：
--
通讯作者：
T. Donohue
中科院分区：
文献类型：
--
作者：
R. Karls;J. Brooks;P. Rossmeissl;J. Luedke;T. Donohue
摘要我们报道了兼性光合细菌类球红细菌的一个基因（rpoH）的作用，该基因编码一种类似于大肠杆菌Escherichia coli E R. sphaeroides β 37控制在环境胁迫下发挥功能的基因，因为anR. sphaeroides ΔRpoH突变体对毒性氧阴离子亚碲酸盐的敏感性比野生型细胞高约30倍。然而，ΔRpoH突变体缺乏E.大肠杆菌细胞缺乏p32。例如，anR.类球孢菌ΔRpoH突变体在噬菌体形态发生中通常没有缺陷，因为它与其野生型亲本一样，也能接种裂解病毒RS 1。在表征R.通过对sphaeroides的生长温度曲线的研究，我们发现当细胞通过有氧呼吸、无氧呼吸或光合作用产生能量时，其生长温度曲线是不同的。然而，在这些条件下，ΔRpoH突变体的生长与野生型菌株的生长相当。当有氧生长的细胞从30 ° C转移到42°C时，ΔRpoH突变体会产生热休克反应，但它表现出120-、85-、75-和65-kDa蛋白质的诱导动力学改变。几种推测的热休克转录物（rpoD PHS，groESL 1等）的积累也减少。当有氧生长的ΔRpoH细胞置于42°C时。在有氧条件下，似乎另一个sigma因子使ΔRpoH突变体能够产生热休克反应，因为来自ΔRpoH突变体的RNA聚合酶制剂，重组的E β 37，或含有38 kDa蛋白质的全酶（E β 38），每个转录E。coliE p32依赖性启动子。光合细胞较低的生长温度与热诱导基因表达的差异相关，因为野生型细胞或ΔRpoH突变体在这种培养物从30 ° C转移到37°C后都不会产生典型的热休克反应。
ABSTRACT We report the role of a gene (rpoH) from the facultative phototroph Rhodobacter sphaeroides that encodes a protein (ς37) similar to Escherichia coliς32 and other members of the heat shock family of eubacterial sigma factors. R. sphaeroides ς37controls genes that function during environmental stress, since anR. sphaeroides ΔRpoH mutant is ∼30-fold more sensitive to the toxic oxyanion tellurite than wild-type cells. However, the ΔRpoH mutant lacks several phenotypes characteristic of E. coli cells lacking ς32. For example, anR. sphaeroides ΔRpoH mutant is not generally defective in phage morphogenesis, since it plates the lytic virus RS1, as well as its wild-type parent. In characterizing the response ofR. sphaeroides to heat, we found that its growth temperature profile is different when cells generate energy by aerobic respiration, anaerobic respiration, or photosynthesis. However, growth of the ΔRpoH mutant is comparable to that of a wild-type strain under each of these conditions. The ΔRpoH mutant mounted a heat shock response when aerobically grown cells were shifted from 30 to 42°C, but it exhibited altered induction kinetics of ∼120-, 85-, 75-, and 65-kDa proteins. There was also reduced accumulation of several presumed heat shock transcripts (rpoD PHS,groESL1, etc.) when aerobically grown ΔRpoH cells were placed at 42°C. Under aerobic conditions, it appears that another sigma factor enables the ΔRpoH mutant to mount a heat shock response, since either RNA polymerase preparations from an ΔRpoH mutant, reconstituted Eς37, or a holoenzyme containing a 38-kDa protein (ς38) each transcribed E. coliEς32-dependent promoters. The lower growth temperature profile of photosynthetic cells is correlated with a difference in heat-inducible gene expression, since neither wild-type cells or the ΔRpoH mutant mount a typical heat shock response after such cultures were shifted from 30 to 37°C.
登录
查看更多内容
热休克 RNA 聚合酶 (E sigma 32) 体外转录大肠杆菌 rrnB P1 启动子。
影响因子：
3.2
作者：
Newlands,JT;Gaal,T;Mecsas,J;Gourse,RL
通讯作者：
Gourse,RL
影响因子：
10.5
作者：
ERICKSON, JW;GROSS, CA
通讯作者：
GROSS, CA
δ-氨基乙酰丙酸将 cycA 转录与球形红杆菌中血红素可用性的变化结合起来。
影响因子：
5.6
作者：
Schilke,BA;Donohue,TJ
通讯作者：
Donohue,TJ
来自球形红杆菌的细胞色素 b-c1 复合物的亚基 IV (Mr = 14,384)。
DOI：
--
发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Usui,S;Yu,L
通讯作者：
Yu,L
红螺菌科遗传技术。
影响因子：
--
作者：
Donohue,TJ;Kaplan,S
通讯作者：
Kaplan,S