The genomics of ecological flexibility, large brains, and long lives in capuchin monkeys revealed with fecalFACS.
The genomics of ecological flexibility, large brains, and long lives in capuchin monkeys revealed with fecalFACS.
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DOI：
10.1073/pnas.2010632118
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发表时间：
2021-02-16
影响因子：
11.1
通讯作者：
Melin AD
中科院分区：
文献类型：
--
作者：
Orkin JD;Montague MJ;Tejada-Martinez D;de Manuel M;Del Campo J;Cheves Hernandez S;Di Fiore A;Fontsere C;Hodgson JA;Janiak MC;Kuderna LFK;Lizano E;Martin MP;Niimura Y;Perry GH;Valverde CS;Tang J;Warren WC;de Magalhães JP;Kawamura S;Marquès-Bonet T;Krawetz R;Melin AD
在充满挑战的环境中生存，长寿，参与复杂的认知过程是人类的标志性特征。在卷尾猴中，类似的特征也在平行进化，但它们的遗传基础仍未被探索。我们开发和注释了一个参考组件的白脸卷尾猴，以探索这些表型的演变。通过比较居住在热带雨林和干燥森林的卷尾猴种群，我们发现了与肾功能，肌肉萎缩和新陈代谢相关的基因选择，这表明适应周期性资源稀缺。当将卷尾猴与其他哺乳动物进行比较时，我们发现了与长寿和大脑发育有关的多个基因的选择证据。我们的研究通过我们的方法来促进，从非侵入性生物材料中生成高覆盖率和低覆盖率的基因组。生态的灵活性、延长的寿命和大的大脑一直吸引着进化生物学家，而比较基因组学提供了一种有效的工具，可以对这些特征的进化产生新的见解。对卷尾猴的研究特别适合阐明选择压力和当地适应不同栖息地、长寿和大脑发育的遗传基础。它们广泛分布在中美洲和南美洲，是富有创造力和提取性的觅食者，以其感觉运动智能而闻名。卷尾猴是所有猴子中相对脑容量最大的，尽管它们的体型很小（3到5公斤），但寿命超过50年。我们组装和注释了一个从头参考基因组的Cebus模仿。通过荧光激活细胞分选（fecalFACS）对来自血液、各种组织和粪便的DNA进行深度测序，分离猴上皮细胞，我们比较了热带干旱森林和低地雨林卷尾猴种群的基因组，并确定了涉及水平衡、肾功能和代谢的基因的种群差异。通过比较基因组学的方法跨越了广泛的哺乳动物的多样性，我们确定了基因的积极选择与长寿和大脑发育。此外，我们提供了一个技术进步，在使用非侵入性基因组学的研究自由放养的哺乳动物。我们的卷尾猴基因组学的种内和种间比较研究提供了深入了解当地适应多样化和生理挑战性环境的过程，以及大脑进化和长寿的分子基础。
Surviving challenging environments, living long lives, and engaging in complex cognitive processes are hallmark human characteristics. Similar traits have evolved in parallel in capuchin monkeys, but their genetic underpinnings remain unexplored. We developed and annotated a reference assembly for white-faced capuchin monkeys to explore the evolution of these phenotypes. By comparing populations of capuchins inhabiting rainforest versus dry forests with seasonal droughts, we detected selection in genes associated with kidney function, muscular wasting, and metabolism, suggesting adaptation to periodic resource scarcity. When comparing capuchins to other mammals, we identified evidence of selection in multiple genes implicated in longevity and brain development. Our research was facilitated by our method to generate high- and low-coverage genomes from noninvasive biomaterials. Ecological flexibility, extended lifespans, and large brains have long intrigued evolutionary biologists, and comparative genomics offers an efficient and effective tool for generating new insights into the evolution of such traits. Studies of capuchin monkeys are particularly well situated to shed light on the selective pressures and genetic underpinnings of local adaptation to diverse habitats, longevity, and brain development. Distributed widely across Central and South America, they are inventive and extractive foragers, known for their sensorimotor intelligence. Capuchins have among the largest relative brain size of any monkey and a lifespan that exceeds 50 y, despite their small (3 to 5 kg) body size. We assemble and annotate a de novo reference genome for Cebus imitator. Through high-depth sequencing of DNA derived from blood, various tissues, and feces via fluorescence-activated cell sorting (fecalFACS) to isolate monkey epithelial cells, we compared genomes of capuchin populations from tropical dry forests and lowland rainforests and identified population divergence in genes involved in water balance, kidney function, and metabolism. Through a comparative genomics approach spanning a wide diversity of mammals, we identified genes under positive selection associated with longevity and brain development. Additionally, we provide a technological advancement in the use of noninvasive genomics for studies of free-ranging mammals. Our intra- and interspecific comparative study of capuchin genomics provides insights into processes underlying local adaptation to diverse and physiologically challenging environments, as well as the molecular basis of brain evolution and longevity.
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世界灵长类动物即将面临的灭绝危机：为什么灵长类动物很重要。
影响因子：
13.6
作者：
Estrada A;Garber PA;Rylands AB;Roos C;Fernandez-Duque E;Di Fiore A;Nekaris KA;Nijman V;Heymann EW;Lambert JE;Rovero F;Barelli C;Setchell JM;Gillespie TR;Mittermeier RA;Arregoitia LV;de Guinea M;Gouveia S;Dobrovolski R;Shanee S;Shanee N;Boyle SA;Fuentes A;MacKinnon KC;Amato KR;Meyer AL;Wich S;Sussman RW;Pan R;Kone I;Li B
通讯作者：
Li B
影响因子：
1.9
作者：
FEDIGAN, LM
通讯作者：
FEDIGAN, LM
DOI：
10.1093/brain/aws312
发表时间：
2013-01
期刊：
Brain : a journal of neurology
影响因子：
--
作者：
Cirak S;Foley AR;Herrmann R;Willer T;Yau S;Stevens E;Torelli S;Brodd L;Kamynina A;Vondracek P;Roper H;Longman C;Korinthenberg R;Marrosu G;Nürnberg P;UK10K Consortium;Michele DE;Plagnol V;Hurles M;Moore SA;Sewry CA;Campbell KP;Voit T;Muntoni F
通讯作者：
Muntoni F
影响因子：
6.9
作者：
Cyranka, Malgorzata;Veprik, Anna;de Wet, Heidi
通讯作者：
de Wet, Heidi
影响因子：
3.5
作者：
Barrett, Brendan J.;Monteza-Moreno, Claudio M.;Crofoot, Margaret C.
通讯作者：
Crofoot, Margaret C.