Differing prevalence and diversity of bacterial species in fetal membranes from very preterm and term labor.
Differing prevalence and diversity of bacterial species in fetal membranes from very preterm and term labor.
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DOI：
10.1371/journal.pone.0008205
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发表时间：
2009-12-08
期刊：
影响因子：
3.7
通讯作者：
Peebles D
中科院分区：
文献类型：
--
作者：
Jones HE;Harris KA;Azizia M;Bank L;Carpenter B;Hartley JC;Klein N;Peebles D
宫内感染可能在自发性早产（PTL）和早产胎膜早破（PPROM）引起的早产中发挥作用。由于以前与早产相关的细菌通常难以培养，因此采用分子生物学方法来鉴定胎盘和胎膜中的细菌 DNA。我们使用广泛的 16S rDNA PCR 和物种特异性实时检测来扩增胎膜和胎盘中的细菌 DNA。 74 名女性被招募到以下组：未足月胎未满 32 周（n = 26；11 例剖腹产）；胎膜完整的 PTL <32 周（n = 19；全部阴道分娩）；表明早产 <32 周（n = 8；全部剖腹产）；足月（n = 21；11 次剖腹产）。 50% (5/10) 的足月阴道分娩细菌 DNA 呈阳性。然而，通过组织观察到的传播很少，并且物种多样性受到限制。在足月选择性部分或指示早产时检测到极少量的细菌。膜完整的 PTL 样本中的细菌流行率显着增加 [足月阴道分娩时为 89% (17/19) 对比 50% (5/10)，p = 0.03] 和 PPROM (CS) [足月选择性 CS 中为 55% (6/11) 对比 0% (0/11)，p = 0.01]。此外，早产组的细菌传播和多样性更大，68% (13/19) 的 PTL 组有 3 个或更多阳性样本，超过 60% (12/19) 显示两种或更多细菌种类（足月阴道分娩的比例为 20% (2/10)）。具有完整膜和 PPROM 且 16S 细菌阳性的 PTL 女性的血单核细胞显示出更高水平的免疫麻痹 (p = 0.03)。 PCR 结果呈阳性与早产组织学绒毛膜羊膜炎相关。早产儿和足月胎膜中都存在细菌。在严重早产的妇女的组织中发现了更大范围的细菌种类和多样性。目前尚不清楚在所有阴道分娩组中观察到的较高细菌流行率在多大程度上反映了分娩过程中细菌污染或膜定植。细菌阳性的早产组织与组织学绒毛膜羊膜炎和明显的母体免疫麻痹有关。
Intrauterine infection may play a role in preterm delivery due to spontaneous preterm labor (PTL) and preterm prolonged rupture of membranes (PPROM). Because bacteria previously associated with preterm delivery are often difficult to culture, a molecular biology approach was used to identify bacterial DNA in placenta and fetal membranes. We used broad-range 16S rDNA PCR and species-specific, real-time assays to amplify bacterial DNA from fetal membranes and placenta. 74 women were recruited to the following groups: PPROM <32 weeks (n = 26; 11 caesarean); PTL with intact membranes <32 weeks (n = 19; all vaginal birth); indicated preterm delivery <32 weeks (n = 8; all caesarean); term (n = 21; 11 caesarean). 50% (5/10) of term vaginal deliveries were positive for bacterial DNA. However, little spread was observed through tissues and species diversity was restricted. Minimal bacteria were detected in term elective section or indicated preterm deliveries. Bacterial prevalence was significantly increased in samples from PTL with intact membranes [89% (17/19) versus 50% (5/10) in term vaginal delivery p = 0.03] and PPROM (CS) [55% (6/11) versus 0% (0/11) in term elective CS, p = 0.01]. In addition, bacterial spread and diversity was greater in the preterm groups with 68% (13/19) PTL group having 3 or more positive samples and over 60% (12/19) showing two or more bacterial species (versus 20% (2/10) in term vaginal deliveries). Blood monocytes from women with PTL with intact membranes and PPROM who were 16S bacterial positive showed greater level of immune paresis (p = 0.03). A positive PCR result was associated with histological chorioamnionitis in preterm deliveries. Bacteria are found in both preterm and term fetal membranes. A greater spread and diversity of bacterial species were found in tissues of women who had very preterm births. It is unclear to what extent the greater bacterial prevalence observed in all vaginal delivery groups reflects bacterial contamination or colonization of membranes during labor. Bacteria positive preterm tissues are associated with histological chorioamnionitis and a pronounced maternal immune paresis.
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影响因子：
1.8
作者：
Gomez, Ricardo;Romero, Roberto;Rojas, Ivan
通讯作者：
Rojas, Ivan
影响因子：
9.8
作者：
Feikin, DR;Thorsen, P;Schuchat, A
通讯作者：
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影响因子：
9.4
作者：
Fredricks, David N.;Fiedler, Tina L.;Marrazzo, Jeanne M.
通讯作者：
Marrazzo, Jeanne M.
影响因子：
9.8
作者：
Hutzal, Carolyn E.;Boyle, Elaine M.;Kirpalani, Haresh
通讯作者：
Kirpalani, Haresh
DOI：
10.1111/j.1749-6632.1999.tb07878.x
发表时间：
1999-01-01
期刊：
NEUROPEPTIDES: STRUCTURE AND FUNCTION IN BIOLOGY AND BEHAVIOR
影响因子：
--
作者：
Hobel, CJ;Arora, CP;Korst, LM
通讯作者：
Korst, LM