Distinct distal gut microbiome diversity and composition in healthy children from Bangladesh and the United States.
Distinct distal gut microbiome diversity and composition in healthy children from Bangladesh and the United States.
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DOI：
10.1371/journal.pone.0053838
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Singh U
中科院分区：
文献类型：
--
作者：
Lin A;Bik EM;Costello EK;Dethlefsen L;Haque R;Relman DA;Singh U
我们目前对人类远端肠道微生物群的组成和稳定性的理解主要基于对生活在发达国家的婴儿和成人的研究。相比之下，人们对年龄较大的儿童和青少年的肠道微生物群及其随时间的变化知之甚少，特别是在发展中国家。我们比较了生活在孟加拉国城市贫民窟的9至14岁健康儿童的粪便微生物群的多样性，组成和时间稳定性，以及美国中上阶层郊区社区相同年龄范围的儿童。我们分析了超过8，000个近全长16 S rRNA基因序列和超过845，000个16 S rRNA V1-V3区域的焦磷酸测序读数。孟加拉国儿童的远端肠道比美国儿童具有更大的细菌多样性，包括来自几个细菌门的新谱系。孟加拉国和美国儿童有不同的粪便细菌群落成员和结构;孟加拉国儿童的微生物群富含普雷沃氏菌、丁酸弧菌和颤螺旋菌，而相对于美国儿童（尽管与孟加拉国成年人相似），拟杆菌减少。此外，孟加拉国儿童的社区成员和结构每月的稳定性明显低于美国儿童。总之，这些结果表明，不同的环境或遗传因素可能会影响这两个国家健康儿童的微生物群。有必要进行进一步调查，以了解这些差异背后的机制和因素，并将这些发现纳入预防和治疗儿童和青少年疾病的新战略。
Our current understanding of the composition and stability of the human distal gut microbiota is based largely on studies of infants and adults living in developed countries. In contrast, little is known about the gut microbiota and its variation over time in older children and adolescents, especially in developing countries. We compared the diversity, composition, and temporal stability of the fecal microbiota of healthy children, ages 9 to 14 years, living in an urban slum in Bangladesh with that of children of the same age range in an upper-middle class suburban community in the United States. We analyzed >8,000 near full-length 16S rRNA gene sequences and over 845,000 pyrosequencing reads of the 16S rRNA V1–V3 region. The distal gut of Bangladeshi children harbored significantly greater bacterial diversity than that of U.S. children, including novel lineages from several bacterial phyla. Bangladeshi and U.S. children had distinct fecal bacterial community membership and structure; the microbiota of Bangladeshi children was enriched in Prevotella, Butyrivibrio, and Oscillospira and depleted in Bacteroides relative to U.S. children (although similar to Bangladeshi adults). Furthermore, community membership and structure in Bangladeshi children was significantly less stable month-to-month than U.S. children. Together, these results suggest that differing environmental or genetic factors may shape the microbiota of healthy children in the two countries. Further investigation is necessary to understand the mechanisms and factors that underlie these differences, and to incorporate these findings into new strategies for the prevention and treatment of childhood and adolescent diseases.
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DOI：
10.1073/pnas.1004204107
发表时间：
2010-08-17
影响因子：
11.1
作者：
Grice, Elizabeth A.;Snitkin, Evan S.;Segre, Julia A.
通讯作者：
Segre, Julia A.
影响因子：
64.8
作者：
Arumugam, Manimozhiyan;Raes, Jeroen;Pelletier, Eric;Le Paslier, Denis;Yamada, Takuji;Mende, Daniel R.;Fernandes, Gabriel R.;Tap, Julien;Bruls, Thomas;Batto, Jean-Michel;Bertalan, Marcelo;Borruel, Natalia;Casellas, Francesc;Fernandez, Leyden;Gautier, Laurent;Hansen, Torben;Hattori, Masahira;Hayashi, Tetsuya;Kleerebezem, Michiel;Kurokawa, Ken;Leclerc, Marion;Levenez, Florence;Manichanh, Chaysavanh;Nielsen, H. Bjorn;Nielsen, Trine;Pons, Nicolas;Poulain, Julie;Qin, Junjie;Sicheritz-Ponten, Thomas;Tims, Sebastian;Torrents, David;Ugarte, Edgardo;Zoetendal, Erwin G.;Wang, Jun;Guarner, Francisco;Pedersen, Oluf;de Vos, Willem M.;Brunak, Soren;Dore, Joel;Weissenbach, Jean;Ehrlich, S. Dusko;Bork, Peer
通讯作者：
Bork, Peer
影响因子：
3.6
作者：
Campbell, DI;Lunn, PG;Elia, M
通讯作者：
Elia, M
影响因子：
5.9
作者：
FAITH, DP
通讯作者：
FAITH, DP
影响因子：
4.4
作者：
Ashelford, Kevin E.;Chuzhanova, Nadia A.;Weightman, Andrew J.
通讯作者：
Weightman, Andrew J.