Assessments of different inactivating reagents in formulating transmissible gastroenteritis virus vaccine.
Assessments of different inactivating reagents in formulating transmissible gastroenteritis virus vaccine.
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DOI：
10.1186/s12985-020-01433-8
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发表时间：
2020-10-23
期刊：
影响因子：
4.8
通讯作者：
Wei Z
中科院分区：
文献类型：
--
作者：
Zhao F;Liu L;Xu M;Shu X;Zheng L;Wei Z
猪传染性胃肠炎病毒（TGEV）引起仔猪肠道感染，以呕吐、严重腹泻和脱水为特征，哺乳仔猪死亡率高达100%。疫苗接种是控制TGEV的有效措施。本研究分别用甲醛（FA）、β-丙内酯（BPL）和二芳基乙烯亚胺（BEI）对细胞培养的TGEV HN-2012株进行灭活。用弗氏佐剂制备TGEV灭活疫苗，并进行免疫效果评价。ELISA法检测TGEV特异性IgG水平。流式细胞术检测CD 4+、CD 8+、CD 4 +IFN-γ+、CD 4 +IL-4+ T淋巴细胞的阳性率。通过淋巴细胞增殖试验、大体病理学和组织病理学检查，对三种灭活剂在TGEV疫苗制备中的作用进行了评价。结果表明，FA组（n = 17）产生TGEV特异性IgG的时间较早且较强，而BEI组产生TGEV特异性IgG的时间较长。淋巴细胞增殖试验表明，BEI组诱导脾淋巴细胞增殖的能力较强。流式细胞术检测外周血淋巴细胞CD 4+、CD 8 + T淋巴细胞亚群阳性率BEI组高于FA组和BPL组。BPL组CD 4 +IFN-γ+ T淋巴细胞亚群阳性率最高，FA组CD 4 +IL-4+ T淋巴细胞亚群阳性率最高。免疫组小鼠和对照组小鼠肉眼及组织病理学检查均未见明显病理变化。结果表明，三个实验组均能诱导小鼠产生细胞免疫和体液免疫，其中FA组的体液免疫效果最好，BEI组的细胞免疫效果最好。
Transmissible gastroenteritis virus (TGEV) causes enteric infection in piglets, characterized by vomiting, severe diarrhea and dehydration, and the mortality in suckling piglets is often high up to 100%. Vaccination is an effective measure to control the disease caused by TGEV. In this study, cell-cultured TGEV HN-2012 strain was inactivated by formaldehyde (FA), β-propiolactone (BPL) or binaryethylenimine (BEI), respectively. Then the inactivated TGEV vaccine was prepared with freund's adjuvant, and the immunization effects were evaluated in mice. The TGEV-specific IgG level was detected by ELISA. The positive rates of CD4+, CD8+, CD4+IFN-γ+, CD4+IL-4+ T lymphocytes were detected by flow cytometry assay. Lymphocyte proliferation assay and gross pathology and histopathology examination were also performed to assess the three different inactivating reagents in formulating TGEV vaccine. The results showed that the TGEV-specific IgG level in FA group (n = 17) was earlier and stronger, while the BEI group produced much longer-term IgG level. The lymphocyte proliferation test demonstrated that the BEI group had a stronger ability to induce spleen lymphocyte proliferation. The positive rates of CD4+ and CD8+ T lymphocyte subsets of peripheral blood lymphocyte in BEI group was higher than that in FA group and BPL groups by flow cytometry assay. The positive rate of CD4+IFN-γ+ T lymphocyte subset was the highest in the BPL group, and the positive rate of CD4+IL-4+ T lymphocyte subset was the highest in the FA group. There were no obvious pathological changes in the vaccinated mice and the control group after the macroscopic and histopathological examination. These results indicated that all the three experimental groups could induce cellular and humoral immunity, and the FA group had the best humoral immunity effect, while the BEI group showed its excellent cellular immunity effect.
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影响因子：
0.4
作者：
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通讯作者：
Gao, M.
影响因子：
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作者：
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DOI：
10.1016/j.nano.2018.02.010
发表时间：
2018-06-01
影响因子：
5.4
作者：
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通讯作者：
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