OfSPL11 Gene from Osmanthus fragrans Promotes Plant Growth and Oxidative Damage Reduction to Enhance Salt Tolerance in Arabidopsis
OfSPL11 Gene from Osmanthus fragrans Promotes Plant Growth and Oxidative Damage Reduction to Enhance Salt Tolerance in Arabidopsis
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桂花的 OfSPL11 基因促进拟南芥植物生长和减少氧化损伤，增强耐盐性
DOI：
10.3390/horticulturae8050412
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发表时间：
2022-05
期刊：
影响因子：
3.1
通讯作者：
赵宏波
中科院分区：
文献类型：
--
作者：
朱守阔;方遒;王艺光;钟诗蔚;董彬;赵宏波
桂花是一种受欢迎的中国传统装饰植物。盐度是影响O.香。然而，在盐胁迫反应中的鳞状细胞启动子结合蛋白样（SPL）基因的参与知之甚少。为了阐明OfSPL基因在盐胁迫抗性中的作用，我们从O.桂花基因型为‘艳红桂’。OfSPL 11是位于细胞核中的转录激活因子。OfSPL 11是盐诱导基因，根据组织特异性表达和外部处理，其在幼叶和芽中高度表达。在烟草叶片和烟草愈伤组织中，盐处理激活了OfSPL 11的启动子活性。香。OfSPL 11转基因株系表现出更好的生长和生理性能;在盐胁迫下，转基因株系具有比野生型（WT）更快的发芽率、更长的根和更少的叶片枯萎。此外，OfSPL 11过表达通过抑制拟南芥中丙二醛（MDA）和活性氧（ROS）的积累来保护叶片免受氧化损伤。OfSPL 11的过表达可以促进一些响应非生物胁迫的基因的表达，包括AtCBL 1，AtCOR 15 A，AtCOR6.6，AtRD 29 A，AtSOS 2和AtSOS 3。酵母单杂交试验和瞬时表达试验表明，OfZAT 12（与拟南芥AtRHL 41基因同源）特异性结合OfSPL 11启动子并正调控其表达。该研究为阐明OfSPL 11在提高O. fragrans通过促进生长和减少氧化损伤。
Osmanthus fragrans Lour. is a popular and traditional Chinese decorative plant. Salinity is one of the major abiotic stresses affecting the growth and development of O. fragrans. However, the involvement of the SQUAMOSA PROMOTER BINDING PROTEIN-like (SPL) gene in salt stress response is little understood. To elucidate the role of the OfSPL genes in salt stress resistance, we isolated a candidate gene, OfSPL11, from the O. fragrans genotype ‘Yanhong Gui’. OfSPL11 is a transcriptional activator that is located in the nucleus. OfSPL11 is a salt-inducible gene that is highly expressed in young leaves and shoots, according to tissue-specific expression and external treatment. The promoter activity of OfSPL11 is activated by salt treatments in the leaves of tobacco and callus of O. fragrans. The OfSPL11 transgenic lines exhibited better growth and physiological performance; under salt stress, transgenic lines have a faster germination rate, longer roots, and less leaf withering than the wild type (WT). In addition, OfSPL11 overexpression protected the leaves from oxidative damage by suppressing the accumulation of malondialdehyde (MDA) and reactive oxygen species (ROSs) in Arabidopsis. OfSPL11 overexpression can promote the expression of some genes in response to abiotic stresses, including AtCBL1, AtCOR15A, AtCOR6.6, AtRD29A, AtSOS2 and AtSOS3. Yeast one-hybrid assays and transient expression assays showed that OfZAT12 (homologous to Arabidopsis AtRHL41 gene) specifically binds to the OfSPL11 promoter and positively regulates its expression. This study sheds fresh light on the role of OfSPL11 in enhancing salt tolerance in O. fragrans by promoting growth and reducing oxidative damage.
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作者：
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