The effects of rhGM‐CSF on the neutrophil respiratory burst when studied in whole blood
The effects of rhGM‐CSF on the neutrophil respiratory burst when studied in whole blood
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在全血中研究时rhGM-CSF对中性粒细胞呼吸爆发的影响
DOI：
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发表时间：
1990
影响因子：
6.5
通讯作者：
D. Linch
中科院分区：
文献类型：
--
作者：
M. Jaswon;A. Khwaja;P. Roberts;H. Jones;D. Linch
建立了一种简便的半定量细胞荧光法测定全血中性粒细胞呼吸爆发活性。该技术避免了引入调节中性粒细胞功能的实验室人工制品。此外，流式细胞术分析允许在单个细胞中研究反应。我们在这里发现，离体检测的中性粒细胞在受到趋化肽FMLP（10− 6 m）刺激时，仅表现出微弱的呼吸爆发活性。预先与rhGM-CSF孵育导致应答细胞数量从13.5±2.36%（平均值±SEM）增加至46.7±6.3%（P<0.0001），总呼吸爆发活性增加567%（P=0.001）。全血中的大多数中性粒细胞（67.1±8.1%）对佛波酯（1 μg/ml TPA）刺激表现出呼吸爆发活性，rhGM-CSF也显著引发了这种反应（P=0.004）。rhGM-CSF诱导的呼吸爆发活性增强是由于先前无应答的中性粒细胞的募集和应答细胞的应答增强。
A simple semiquantitative cytofluorometric method has been developed for measuring neutrophil respiratory burst activity in whole blood samples. This technique avoids the introduction of laboratory artefacts which modulate neutrophil function. In addition, flow cytometric analysis allows the response to be studied in individual cells. We show here that neutrophils examined freshly ex vivo, exhibit only weak respiratory burst activity in response to stimulation with the chemotactic peptide FMLP (10−6m). Prior incubation with rhGM‐CSF results in an increase in the number of responding cells from 13.5±2.36% (mean ±SEM) to 46.7±6.3% (P<0.0001) with an increase in total respiratory burst activity of 567% (P=0.001). The majority of neutrophils in whole blood (67.1±8.1%) exhibit respiratory burst activity in response to stimulation with phorbol ester (1 μg/ml of TPA), and this response is also significantly primed by rhGM‐CSF (P=0.004). The enhancement of respiratory burst activity induced by rhGM‐CSF is due to both recruitment of previously unresponsive neutrophils, and to intensification of the response of the responding cells.
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影响因子：
20.3
作者：
Ann M. Dvorak;L. Letourneau;G. Login;Peter F. Weller;Steven J. Ackerman
通讯作者：
Ann M. Dvorak;L. Letourneau;G. Login;Peter F. Weller;Steven J. Ackerman
影响因子：
4.4
作者：
D. English;H. Broxmeyer;T. Gabig;L. Akard;D. Williams;R. Hoffman
通讯作者：
D. English;H. Broxmeyer;T. Gabig;L. Akard;D. Williams;R. Hoffman
影响因子：
15.9
作者：
LOPEZ, AF;WILLIAMSON, DJ;VADAS, MA
通讯作者：
VADAS, MA
粒细胞-巨噬细胞集落刺激因子增强中性粒细胞功能涉及招募反应较弱的亚群。
影响因子：
20.3
作者：
Fletcher,MP;Gasson,JC
通讯作者：
Gasson,JC
影响因子：
4.4
作者：
D. Bass;J. W. Parce;L. Dechatelet;P. Szejda;M. Seeds;M. Thomas
通讯作者：
D. Bass;J. W. Parce;L. Dechatelet;P. Szejda;M. Seeds;M. Thomas