Invasion of E. coli biofilms by antibiotic resistance plasmids.
Invasion of E. coli biofilms by antibiotic resistance plasmids.
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DOI：
10.1016/j.plasmid.2013.03.003
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发表时间：
2013-07
期刊：
影响因子：
2.6
通讯作者：
Top EM
中科院分区：
文献类型：
--
作者：
Król JE;Wojtowicz AJ;Rogers LM;Heuer H;Smalla K;Krone SM;Top EM
尽管质粒对人类病原体中抗生素耐药性的传播有贡献，但对细菌生物膜中各种耐药质粒的可转移性知之甚少。本研究的目的是比较19种多药耐药质粒在大肠杆菌中的转移效率。大肠杆菌受体生物膜，并确定生物膜年龄，生物膜供体暴露时间，和供体对生物膜附着在这个过程中的影响。大肠coli受体生物膜分别暴露于19株E.通过平板计数确定每个具有不同质粒的大肠杆菌供体和转移接合物。除了少数例外，在液体环境中转移良好的质粒在生物膜中也表现出最高的可转移性。最大和最小可转移质粒之间的转移频率差异几乎是百万倍。质粒对生物膜的“侵袭性”，或在几小时内获得质粒的生物膜细胞的比例，不仅取决于质粒的类型，还取决于生物膜暴露于供体的时间和质粒供体附着于生物膜的能力，而不是生物膜年龄。供体菌株附着到生物膜的效率不受质粒存在的影响。最具侵袭性的质粒是pHH 2 -227，基于基因组序列分析，其是IncU样质粒和IncW质粒的杂交体。E.质粒之间的大肠杆菌生物膜需要考虑到我们在打击耐药性的传播。
In spite of the contribution of plasmids to the spread of antibiotic resistance in human pathogens, little is known about the transferability of various drug resistance plasmids in bacterial biofilms. The goal of this study was to compare the efficiency of transfer of 19 multidrug resistance plasmids into E. coli recipient biofilms and determine the effects of biofilm age, biofilm-donor exposure time, and donor-to-biofilm attachment on this process. An E. coli recipient biofilm was exposed separately to 19 E. coli donors, each with a different plasmid, and transconjugants were determined by plate counting. With few exceptions, plasmids that transferred well in a liquid environment also showed the highest transferability in biofilms. The difference in transfer frequency between the most and least transferable plasmid was almost a million-fold. The ‘invasibility’ of the biofilm by plasmids, or the proportion of biofilm cells that acquired plasmids within a few hours, depended not only on the type of plasmid, but also on the time of biofilm exposure to the donor and on the ability of the plasmid donor to attach to the biofilm, yet not on biofilm age. The efficiency of donor strain attachment to the biofilm was not affected by the presence of plasmids. The most invasive plasmid was pHH2-227, which based on genome sequence analysis is a hybrid between IncU-like and IncW plasmids. The wide range in transferability in an E. coli biofilm among plasmids needs to be taken into account in our fight against the spread of drug resistance.
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影响因子：
4.4
作者：
ANGLES, ML;MARSHALL, KC;GOODMAN, AE
通讯作者：
GOODMAN, AE
影响因子：
2.6
作者：
CULLUM, J;COLLINS, JF;BRODA, P
通讯作者：
BRODA, P
影响因子：
2.8
作者：
Burmolle, Mette;Bahl, Martin Lain;Hansen, Lars Hestbjerg
通讯作者：
Hansen, Lars Hestbjerg
影响因子：
3.5
作者：
Christensen, BB;Sternberg, C;Molin, S
通讯作者：
Molin, S
DOI：
10.1099/00221287-70-3-453
发表时间：
1972-01-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
--
作者：
DATTA, N;HEDGES, RW
通讯作者：
HEDGES, RW