Drosophila Mon2 couples Oskar-induced endocytosis with actin remodeling for cortical anchorage of the germ plasm
Drosophila Mon2 couples Oskar-induced endocytosis with actin remodeling for cortical anchorage of the germ plasm
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果蝇 Mon2 将 Oskar 诱导的内吞作用与肌动蛋白重塑结合起来，以实现种质的皮质锚定
DOI：
10.1242/dev.062208
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发表时间：
2011
期刊：
影响因子：
4.6
通讯作者：
A. Nakamura
中科院分区：
文献类型：
--
作者：
Tsubasa Tanaka;Yasuko Kato;Kazuki Matsuda;Kazuko Hanyu;A. Nakamura
果蝇极（生殖）质包含生殖系和腹部决定因素。它的装配开始于oskar（osk）RNA在卵母细胞后部的定位和翻译，为了正常的胚胎发育，极浆必须限制在卵母细胞后部。Osk刺激内吞作用，这反过来又促进肌动蛋白重塑，在卵母细胞后极形成长的F-肌动蛋白突起。虽然内吞偶联的肌动蛋白重构似乎是极浆锚定的关键，但Osk诱导的内吞活性和肌动蛋白重构之间的联系机制尚不清楚。在这里，我们报告说，高尔基体内体蛋白，蒙2，行为下游的Osk重塑皮质肌动蛋白和锚极浆。Mon 2与已知参与卵母细胞中osk RNA定位的两种肌动蛋白成核剂Cappuccino（Capu）和Spire（Spir）相互作用，并促进小GTdR Rho 1在卵母细胞后部的积累。我们还发现，这些肌动蛋白监管机构所需的OSK依赖形成的长F-肌动蛋白的预测和皮质锚定极等离子体组件。我们建议，在响应Osk介导的内吞激活，囊泡定位的Mon 2作为一个支架，指示肌动蛋白重塑复合物形成长的F-肌动蛋白的预测。这种Mon 2介导的偶联事件对于将极浆限制在卵母细胞后皮质是至关重要的。
Drosophila pole (germ) plasm contains germline and abdominal determinants. Its assembly begins with the localization and translation of oskar (osk) RNA at the oocyte posterior, to which the pole plasm must be restricted for proper embryonic development. Osk stimulates endocytosis, which in turn promotes actin remodeling to form long F-actin projections at the oocyte posterior pole. Although the endocytosis-coupled actin remodeling appears to be crucial for the pole plasm anchoring, the mechanism linking Osk-induced endocytic activity and actin remodeling is unknown. Here, we report that a Golgi-endosomal protein, Mon2, acts downstream of Osk to remodel cortical actin and to anchor the pole plasm. Mon2 interacts with two actin nucleators known to be involved in osk RNA localization in the oocyte, Cappuccino (Capu) and Spire (Spir), and promotes the accumulation of the small GTPase Rho1 at the oocyte posterior. We also found that these actin regulators are required for Osk-dependent formation of long F-actin projections and cortical anchoring of pole plasm components. We propose that, in response to the Osk-mediated endocytic activation, vesicle-localized Mon2 acts as a scaffold that instructs the actin-remodeling complex to form long F-actin projections. This Mon2-mediated coupling event is crucial to restrict the pole plasm to the oocyte posterior cortex.
果蝇第二条染色体上的雌性不育突变。
影响因子：
3.3
作者：
Schüpbach,T;Wieschaus,E
通讯作者：
Wieschaus,E
Rho1 小 GTP 酶的突变会破坏果蝇早期发育过程中的形态发生和分割。
DOI：
10.1242/dev.126.23.5353
发表时间：
1999
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Magie,CR;Meyer,MR;Gorsuch,MS;Parkhurst,SM
通讯作者：
Parkhurst,SM