Regulation of photochemical energy transfer accompanied by structural changes in thylakoid membranes of heat-stressed wheat.
Regulation of photochemical energy transfer accompanied by structural changes in thylakoid membranes of heat-stressed wheat.
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DOI：
10.3390/ijms151223042
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发表时间：
2014-12-11
影响因子：
5.6
通讯作者：
Sugimoto Y
中科院分区：
文献类型：
--
作者：
Marutani Y;Yamauchi Y;Miyoshi A;Inoue K;Ikeda K;Mizutani M;Sugimoto Y
高等植物的光系统在中度胁迫条件下在光存在下减轻热诱导的损伤;然而，在没有光的情况下（即，在黑暗中），同样的植物更容易受损。（Yamauchi和Kimura，2011）我们证明，在40 °C下用光调节热处理小麦的光化学能量转移有助于光系统的耐热性。利用热胁迫小麦幼苗在光照下进行叶绿素荧光分析表明，叶绿素荧光的非光化学猝灭（NPQ）增加，这是由于状态1到状态2的转换增加了热耗散。透射电子显微镜显示类囊体膜的结构变化，包括在光的热应力下的基粒区域的unstacking。它伴随着类囊体蛋白如D1和D2蛋白和捕光复合物II蛋白Lhcb 1和Lhcb 2的磷酸化。这些结果表明，在40 °C的光下的热胁迫诱导状态1到状态2的转变，通过更强烈地磷酸化类囊体蛋白来优先激发光系统I（PSI）。类囊体膜的结构变化也有助于光系统的重塑和向状态2过渡的能量分配的调节可能有助于质体醌氧化，因此，光驱动的电子流经PSI热应激下对PSII损伤发挥保护作用。
Photosystems of higher plants alleviate heat-induced damage in the presence of light under moderate stressed conditions; however, in the absence of light (i.e., in the dark), the same plants are damaged more easily. (Yamauchi and Kimura, 2011) We demonstrate that regulating photochemical energy transfer in heat-treated wheat at 40 °C with light contributed to heat tolerance of the photosystem. Chlorophyll fluorescence analysis using heat-stressed wheat seedlings in light showed increased non-photochemical quenching (NPQ) of chlorophyll fluorescence, which was due to thermal dissipation that was increased by state 1 to state 2 transition. Transmission electron microscopy revealed structural changes in thylakoid membranes, including unstacking of grana regions under heat stress in light. It was accompanied by the phosphorylation of thylakoid proteins such as D1 and D2 proteins and the light harvesting complex II proteins Lhcb1 and Lhcb2. These results suggest that heat stress at 40 °C in light induces state 1 to state 2 transition for the preferential excitation of photosystem I (PSI) by phosphorylating thylakoid proteins more strongly. Structural changes of thylakoid membrane also assist the remodeling of photosystems and regulation of energy distribution by transition toward state 2 probably contributes to plastoquione oxidation; thus, light-driven electrons flowing through PSI play a protective role against PSII damage under heat stress.
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影响因子：
11.6
作者：
Pietrzykowska, Malgorzata;Suorsa, Marjaana;Jansson, Stefan
通讯作者：
Jansson, Stefan
影响因子：
3.7
作者：
Bukhov, NG;Wiese, C;Heber, U
通讯作者：
Heber, U
DOI：
10.1016/0005-2728(90)90018-y
发表时间：
1990-02-02
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
CAO, J;GOVINDJEE
通讯作者：
GOVINDJEE
影响因子：
4.1
作者：
Jarvi, Sari;Suorsa, Marjaana;Aro, Eva-Mari
通讯作者：
Aro, Eva-Mari
DOI：
10.1111/tpj.12297
发表时间：
2013-10
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Leoni C;Pietrzykowska M;Kiss AZ;Suorsa M;Ceci LR;Aro EM;Jansson S
通讯作者：
Jansson S