Very rapid phosphorylation kinetics suggest a unique role for Lhcb2 during state transitions in Arabidopsis.
Very rapid phosphorylation kinetics suggest a unique role for Lhcb2 during state transitions in Arabidopsis.
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DOI：
10.1111/tpj.12297
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发表时间：
2013-10
期刊：
影响因子：
--
通讯作者：
Jansson S
中科院分区：
文献类型：
--
作者：
Leoni C;Pietrzykowska M;Kiss AZ;Suorsa M;Ceci LR;Aro EM;Jansson S
捕光复合物II（LHCII）含有三种高度同源的叶绿素a/b结合蛋白（Lhcb 1、Lhcb 2和Lhcb 3），它们可以组装成同源和异源三聚体。Lhcb 1和Lhcb 2在所谓的状态转换过程中通过STN 7激酶和PPH 1/TAP 38磷酸酶的作用可逆地磷酸化。我们已经开发出对Lhcb 1和Lhcb 2的磷酸化形式具有特异性的抗体。我们发现Lhcb 2比Lhcb 1更快地磷酸化：10秒的“状态2光”导致Lhcb 2磷酸化至最大水平的30%。磷酸化和非磷酸化形式的蛋白质电泳迁移率和去磷酸化动力学没有显着差异，这两种蛋白质之间没有差异。在状态2中，大多数磷酸化形式的Lhcb 1和Lhcb 2存在于超级和巨型复合物，包括光系统（PS）I和PSII，和状态2特异性PSI-LHCII复合物高度富集在磷酸化形式的Lhcb 2。我们的研究结果意味着不同的和具体的作用，Lhcb 1和Lhcb 2在光合捕光的调节。
Light-harvesting complex II (LHCII) contains three highly homologous chlorophyll-a/b-binding proteins (Lhcb1, Lhcb2 and Lhcb3), which can be assembled into both homo- and heterotrimers. Lhcb1 and Lhcb2 are reversibly phosphorylated by the action of STN7 kinase and PPH1/TAP38 phosphatase in the so-called state-transition process. We have developed antibodies that are specific for the phosphorylated forms of Lhcb1 and Lhcb2. We found that Lhcb2 is more rapidly phosphorylated than Lhcb1: 10 sec of ‘state 2 light’ results in Lhcb2 phosphorylation to 30% of the maximum level. Phosphorylated and non-phosphorylated forms of the proteins showed no difference in electrophoretic mobility and dephosphorylation kinetics did not differ between the two proteins. In state 2, most of the phosphorylated forms of Lhcb1 and Lhcb2 were present in super- and mega-complexes that comprised both photosystem (PS)I and PSII, and the state 2-specific PSI–LHCII complex was highly enriched in the phosphorylated forms of Lhcb2. Our results imply distinct and specific roles for Lhcb1 and Lhcb2 in the regulation of photosynthetic light harvesting.
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DOI：
10.1016/0005-2728(88)90248-4
发表时间：
1988-10-26
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
BASSI, R;RIGONI, F;GIACOMETTI, GM
通讯作者：
GIACOMETTI, GM
影响因子：
2.9
作者：
BLUM, H;BEIER, H;GROSS, HJ
通讯作者：
GROSS, HJ
DOI：
10.1016/0005-2728(69)90168-6
发表时间：
1969-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
BONAVENTURA, C;MYERS, J
通讯作者：
MYERS, J
影响因子：
64.8
作者：
Bonardi, V;Pesaresi, P;Leister, D
通讯作者：
Leister, D
影响因子：
7.4
作者：
Ganeteg, U;Strand, Å;Jansson, S
通讯作者：
Jansson, S